14,6 [+ or -] 1,1 Prothrombin time (s) 25,3 [+ or -] 1,2 Thrombin time (s) 48,0 [+ or -] 1,1 Free heparin (s) 9,3 [+ or -] 0,3 Antithrombin III (s) 45,3 [+ or -] 3,5 Fibrinogen (g/liter) 1,9 [+ or -] 0,2 Total euglobulin fibrinolysis (min) 20,0 [+ or -] 0,0 Hageman-dependent fibrinolysis (min) 320,0 [+ or -] 0,0 Ethanol sample 10 (-) Protamine sulfate
sample 10(-) After infiltration of layer (fraction) Indications [OFXG.
At the present time, a substance known as protamine sulfate
is the only agent in the world that is available commercially to reverse the blood-thinning of heparin.
Gene delivery using protamine sulfate
(PS) involves condensation of ON into compact particles, uptake into the cells, release from the endosomal compartment into the cytoplasm, and uptake of the ON into the nucleus.
A comparison of thrombelastography with heparinase or protamine sulfate
added in vitro during heparinized cardiopulmonary bypass.
The effect could be reproduced by adding heparin to serum, and it was largely blocked in heparin plasma by addition of the heparin antagonist protamine sulfate
is the only agent currently commercially available to reverse heparin and its use is associated with numerous side effects as detailed in question number four below.
Alternatively, treatment with thrombin (1) or protamine sulfate
in combination with thrombin (2) has been used to remove the fibrinogen interference, thereby establishing presumptive identification.
The 13th edition of the AABB Technical Manual lists a procedure for the treatment of incompletely clotted specimens using thrombin, 1-percent protamine sulfate
, glass beads, and epsilon aminocaproic acid (EACA), which may be useful if testing facilities are still using clot tubes rather than EDTA.
After extensive washing to remove the papain, the retinas were mechanically agitated through a glass-graduated pipette, and aliquots of the solution containing isolated cells were placed on glass cover slips that had previously been coated with protamine sulfate
and concanavalin A.
These test patients will receive the normal intraoperative dose of heparin but at the end of surgery the Heparin Removal Device will be used to negate the anti-clotting effects of heparin as opposed to the current protamine sulfate
This method uses titration to a given concentration of heparin using either the protamine sulfate
neutralization technique or a factor Xa inhibition chromogenic assay.
(from herring), beef lung heparin, and tris[(hydroxy methyl)amino methane] (Tris) were from Sigma Chemical Co.