pneumonia ,Salmonella typhi isolates and 50% of Proteus vulgaris
and Serratia marcescens isolates as showed in Fig.
epidermidis, Proteus vulgaris
, Streptococcus oralis, and Candida albicans) and clinical isolated methicillin resistant S.
Y Proteus vulgaris
presento resistencia a cefalosporinas (ceftriaxona, cefuroxima) (CMI >16), Tabla 1.
There were 9 (18%) Gramnegative bacillary isolates, including 3 (6%) Escherichia coli; 2 (4%) isolates of Pseudomonas aeruginosa, and one isolate (2%) each of Proteus vulgaris
, Enterobacteraerogenes, Klebsiella, and Haemophilus influenzae [Table-3].
The isolates comprised 112 (58%) Gram-negative organisms which included Proteus vulgaris
, Enterobacter spp., Klebsiella spp., Serratia liquefaciens, and Pseudomonas fluorescens.
Seven human gram negative bacteria Escherichia coli (ATCC 27853), Pseudomonas aeruginosa (ATCC 25922), Klebsiella pneumonia (ATCC 8308), Enterococcus faecalis (ATCC 29212), Shigella flexneri (ATCC 12022), Salmonella typhimurium (ATCC 14028), and Proteus vulgaris
(ATCC 8427) along with one human gram positive bacterium Staphylococcus aureus (ATCC 25923) were maintained on nutrient agar slants that contained peptone (5.0 g), meat extract (1.0 g), yeast extract (2.0 g), sodium chloride (5.0 g), and agar (15.0 g) per liter of distilled water.
The following bacteria were isolated and identified following the methods and techniques described by Barrow and Feltham : Bacillus subtilis, Escherichia coli, Moraxella bovis, Proteus vulgaris
, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes (Group A), and Trueperella (Arcanobacterium) pyogenes (Table 2).
The synthesized complexes were studied for their biological activities against gram negative bacteria including Escherichia coli, Salmonella typhi, Enterobacter aerogenes, Proteus vulgaris
, Pseudomonas aeruginosa, Gram positive bacterial strains like Staphylococcus aureus and fungus like Candida albican.
(11.6% of isolates, 19.7% of individuals), 21 Proteus vulgaris
(4.2% of isolates, 7.0% of individuals), 5 Providencia alcalifaciens (0.98% of isolates, 1.67% of individuals), 5 Serratia sp.
The enzyme Chondroitinase ABC purified from Proteus vulgaris
degrades these CSPGs, and has been shown to promote functional recovery and neural regeneration.
All the compounds were tested for activity against gram-positive bacteria and gram-negative bacteria like Neisseria gonorrhoeae, Proteus vulgaris
, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus.
The pus stained positive for bacteria and acid-fast bacilli, and culture was positive for Proteus vulgaris