- Launch of Thermo Fisher Scientific's New PureLink
Fast Low-Endotoxin Plasmid Purification Midi and Maxi Kits
The PCR products were purified using the PureLink
Quick Gel Extraction Kit (Invitrogen, USA), cloned into the pCR2.1-TOPO vector (Invitrogen, USA), and transformed using Escherichia coli (E.
GmbH has combined Semtech's BlueRiver Software Defined Video over Ethernet (SDVoE) technology with its new IPAV Pro Series products.
The next day, plasmid DNA was extracted from the cultures with the Invitrogen PureLink
Quick Plasmid Miniprep Kit (Carlsbad, CA).
We centrifuged 5 [micro]L blood at 4,000 rpm for 10 min and collected the buffy coat for DNA extraction by using the PureLink
Kit (Invitrogen, https://www.thermofisher.com).
RNA was extracted from the sorted cells using the PureLink
RNA Microkit (cat: 12183_016; Invitrogen, Carlsbad, CA, USA).
The PCR products of rDNA region presenting a single band with the desired length (approximately 500-800pb) were purified with PureLink
PCR Purification Kit (Invitrogen), and the DNA was sequenced in an automatic sequencer (ABIPrism 3500 Genetic Analyzer) using the primers ITS1 and ITS4 (WHITE, 1990).
Total RNA was extracted from each tissue using the PureLink
RNA Mini Kit (Life Technologies) with a TRIzol step.
RNA isolation from cultured neurons was performed with PureLink
RNA Mini Kit (Thermo Fischer 12183018A).
Total RNA was extracted from transfected 293FT cells using PureLink
RNA mini kit (Invitrogen) and the cDNA synthesis was performed with high capacity cDNA reverse transcription kit (Applied Biosystems, USA), according to the manufacturer's instructions.
Genomic DNA of each subject was extracted from EDTA-treated whole blood using the PureLink
Genomic DNA Mini Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
Ramsey, NJ, March 30, 2018 --(PR.com)-- PureLink
, a commercial and residential A/V connectivity solutions provider to professional integrators and consultants worldwide, announced today the use of their equipment by the Philadelphia Flyers organization at their home NHL venue, Wells Fargo Center.