restriction fragment length polymorphism

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restriction fragment length polymorphism

[ri¦strik·shən ¦frag·mənt ‚leŋkth ‚päl·i′mȯr‚fiz·əm]
(cell and molecular biology)
Variations in the length of restriction fragments resulting from action by a specific endonuclease.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs and sequences of ITS-rDNA.
On the basis of RFLP pattern, the unique isolate was subjected to sequence analysis.
These results are conformity (16) who conducted an experiment to know the variation in the internal transcribed spacer by RFLP using the restriction enzymes Rsal, HpaII, Sau3AI, andMspI.
Moreover, this method eliminates the need for postamplification manipulations and technical problems that are sometimes associated with RFLP analyses of amplicons produced by nested PCR (14,15).
Za RFLP secenje koristili smo 2 [micro]l pufera, 10 u HhaI enzima i 7 [micro]l [H.sub.2]O za mastermiks u kiveti od 1,5 ml.
So it is desirable that RFLP of both genes should be used for better and reliable discrimination.
PGRS and DR RFLP. PGRS-RFLP using PvuII was successfully conducted on 62 isolates and we classified them into 25 genotypic groups (P1 to P25) (Figure 1).
RFLP analysis showed 17 different alleles in type B genotype (B1-B17) and 5 alleles in type A genotype (A1-A5) (Table 1).
Despite the high degree of IS6110-associated restriction fragment length polymorphism (RFLP) among M.