Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs
and sequences of ITS-rDNA.
On the basis of RFLP
pattern, the unique isolate was subjected to sequence analysis.
These results are conformity (16) who conducted an experiment to know the variation in the internal transcribed spacer by RFLP
using the restriction enzymes Rsal, HpaII, Sau3AI, andMspI.
Moreover, this method eliminates the need for postamplification manipulations and technical problems that are sometimes associated with RFLP
analyses of amplicons produced by nested PCR (14,15).
secenje koristili smo 2 [micro]l pufera, 10 u HhaI enzima i 7 [micro]l [H.sub.2]O za mastermiks u kiveti od 1,5 ml.
types of original soil showed a rather high variability.
So it is desirable that RFLP
of both genes should be used for better and reliable discrimination.
PGRS and DR RFLP
. PGRS-RFLP using PvuII was successfully conducted on 62 isolates and we classified them into 25 genotypic groups (P1 to P25) (Figure 1).
analysis showed 17 different alleles in type B genotype (B1-B17) and 5 alleles in type A genotype (A1-A5) (Table 1).
Despite the high degree of IS6110-associated restriction fragment length polymorphism (RFLP
) among M.