Prevalence, correlates and clinical usefulness of antibodies to RNA polymerase III
in systemic sclerosis: a cross-sectional analysis of data from an Australian cohort.
Lund et al., "RNA Polymerase III
Subunit POLR3G Regulates Specific Subsets of PolyA and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells," Stem Cell Reports, 2017; 8 (5): 1442 DOI: 10.1016/j.stemcr.2017.04.016
Eukaryotic RNA polymerase III (RNAP III) is one of them and synthesizes tRNA, 5S ribosomal RNA, and other small RNAs (for review [1, 2]).
White, "Transcription by RNA polymerase III: more complex than we thought," Nature Reviews Genetics, vol.
Matsutani, "Similarities in transcription factor IIIC subunits that bind to the posterior regions of internal promoters for RNA polymerase III," BMC Evolutionary Biology, vol.
van der Oost, "Identification of an ortholog of the eukaryotic RNA polymerase III subunit RPC34 in Crenarchaeota and Thaumarchaeota suggests specialization of RNA polymerases for coding and non-coding RNAs in Archaea," Biology Direct, vol.
Werner, "Dual role of the C34 subunit of RNA polymerase III in transcription initiation," The EMBO Journal, vol.
"We identified many different mutations in the POLR3A gene which codes for a key subunit of RNA Polymerase III
(Pol III), a highly conserved protein complex with a crucial role in gene expression, and many other important pathways," Dr.
Importantly this vector has been designed to express siRNAs from the RNA Polymerase III
H1 promoter (13), making it a logical choice for expressing other small RNAs such as hammerhead ribozymes.
The other transcription factors and RNA polymerase III are presumed to be in excess over available TFIIIA, because tRNA genes are transcribed in the egg extract.
First it inhibited transcription generally; this inhibition could be alleviated in part by supplementing the mixture with high concentrations of RNA polymerase III. Second, the egg extract destabilized transcription complexes formed by oocyte but not by somatic 5S genes" (2; italics added).