ABTS is well regarded as a peroxidase substrate that generates a metastable radical cation
(20) After donating an electron to OH* melatonin becomes a free radical itself, the indolyl radical cation
with very low reactivity, thereby being non toxic to cells.
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation
Formation of radical cation
and molecular structure of polyaniline.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical cation
scavenging assay: 40 [micro]L of extracts prepared at different final concentrations (20 [micro]g/mL, 50 [micro]g/mL, 100 [micro]g/mL), 3960 [micro]L of ABTS*+ working solution were combined.
ABTS radical cation
(ABTS*+) was produced by reacting ABTS stock solution with 2.45 mM potassium persulfate (final concentration) and allowing the mixture to stand in the dark at room temperature for 12-16 h before use.
The S[C.sub.50] values (concentration of mango peel extract capable of scavenging 50% of ABTS radical cation
) of the peel extracts (NPEE, CPEE, NPWE, and CPWE) from six mango cultivars for ABTS radical cation-scavenging activity are also presented in Table 2.
Since the characteristic absorption spectrum of ABTS cation radical can be determined at 414, 645, 734, and 815 nm [30, 31] because of the presence of antioxidants , common organic radical cation
([ABTS.sup.+]) assay is one of the most widely applied methods to assess antioxidant activity .
Erel, "A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
," Clinical Biochemistry, vol.
ABTS radical cation
scavenging activity: The ABTS (2, 2 azobis, 3-ethylbenzothiozoline-6-sulphonic acid) radical cation
scavenging activity was performed by a slight modification of Re et al.
Free radical scavenging of the pulp extract was determined by ABTS radical cation
decolorization assay (Re et al., 1999) as adapted by Rufino et al.
In case an amount equivalent to one electron is transferred, the nucleophile becomes a radical cation
, while the electrophile becomes a radical anion.