Raffinose


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Related to Raffinose: Melezitose, stachyose

Raffinose

The best-known trisaccharide (oligosaccharide), widely distributed in higher plants. The best-known sources are cottonseed meal and the manna of Eucalyptus. It is also known as melitose, melitriose, gossypose, and O-α- d -galactopyranosyl-(1→6)-O-α- d -glucopyranosyl-(1→2)-β- d -fructofuranoside. See Oligosaccharide

Complete acid hydrolysis gives 1 mole each of d -galactose, d -glucose, and d -fructose. In structure, it comprises melibiose and sucrose with the central d -glucose in common. See Fructose, Galactose, Glucose

Raffinose can be hydrolyzed by enzymes in two ways. Invertase (β- d -fructofuranoside) hydrolyzes the sucrose part of the molecule to give melibiose and d -fructose. Almond emulsin, which contains an α- d -galactosidase, hydrolyzes the melibiose residue to yield d -galactose and sucrose.

Raffinose was found to be enzymically synthesized in plants from uridine diphosphate d -galactose and sucrose by an enzyme which transfers the d -galactose moiety of this sugar nucleotide to sucrose, resulting in the formation of raffinose.

Raffinose

 

a nonreducing trisaccharide composed of residues of D-galactose, D-glucose, and D-fructose. Raffinose is a colorless water-soluble substance with melting points of 80°C (pentahydrate) and 119°–120°C (anhydrous). It is one of the most widely distributed stored carbohydrates in plants (sugar beet, cottonseed meal, Australian manna). The enzyme a-galactosidase splits raffinose into galactose and the disacchar-ide saccharose, and invertase splits it into fructose and melibiose.

raffinose

[′raf·ə‚nōs]
(biochemistry)
C18H32O16·5H2O A white, crystalline trisaccharide found in sugarbeets, cottonseed meal, and molasses; yields glucose, fructose, and galactose on complete hydrolysis. Also known as gossypose; melitose; melitriose.
References in periodicals archive ?
Leske et al [32] also suggested that the raffinose family of oligosaccharides can cause shortened transit time, which led to a reduced fiber fermentation.
In the present study, the compliance of participants to the study foods in the chickpea period was similar to that of the control period, whereas the raffinose period had the highest compliance.
Beano tablets can prevent gas because they contain an enzyme that breaks down raffinose and stachyose.
The raffinose family oligosaccharides (RFOs), namely ciceritol, raffinose, stachyose, and verbascose are not desirable as usually result in bloating or flatus when consumed due to human inability to digest 1-6 galactosidic linkage [15].
The content of raffinose as a mass fraction of total sugars and the magnitude of the glassy state showed positive correlations with storage stability.
And out of five isolates, four isolates were positive for Sacarose, Trehalose, Raffinose, and Eesculine hydrolysis on the basis of PIBwin software, the Bacillus spp.
The utilized carbon sources are, D-Glucose anhydrous, Lactose monohydrate, Maltose monohydrate, Sucrose, D (+) Manitol, D (+) Raffinose, and D (+) Xylose and, all were obtained from Nice Chemicals Pvt.
Dextrose, sucrose, and raffinose assimilation tests were performed in triplicate by using yeast nitrogen base medium.
Honeydew honey is characterized by a higher concentration of oligosaccharides, mainly trisaccharides melezitose and raffinose, which usually are not found in blossom honeys [7,8].
In contrast Rhizopus oligosporus did not produces a-galactosidase and consequently can not degrade flatulence-causing compounds such as stachyose and raffinose [23].
The biochemical tests used to identify enterococci species was Purple Broth Base (Difco, Detroit, USA) supplemented with arabinos, MGP (Metil-[alpha]-Dglycopiranoside), arginine, mannitol, sorbitol, sorbose and raffinose.