The universal primers used for amplifying these four DNA regions were as follows: rbcL
, 1F and 724R (Fay et al.
Support for an expanded family concept of Malvaceae within recircumscribed order Malvales: a combined analysis of plastid atpB and rbcL
Systematics of the Eriacaceae, Empetraceae, Epacridaceae and related taxa based on up rbcL
Specimens analysed directly by authors and their GenBank accessions (rps16 intron, trnL intron and trnL-trnF intergenic spacer, atpB-rbcL
intergenic spacer, rbcL
with a part of rbcL
-accD intergenic spacer, and partial trnK intron and matK).
2002) as rbcL
sequences were moved to the ndhF matrix as they correspond to that gene, (3) the inverse (reversed complement) sequence for accession number Z00044 (Nicotiana tabacum chloroplast genome) was used for the rpsl6 sequence, (4) accession number U73971 for Orobanche racemosa was not used because Bremer et al.
In this course the students will isolate the genomic DNA and amplify chloroplast rbcL
sequences by polymerase chain reaction (PCR).
Reorganization of the genus Psychotria and the tribe Psychotreae (RUBIACEAE) inferred from ITS and rbcl
Phylogenetic relationships in Sarraceniaceae based on rbcL
and ITS sequences.
To determine the dimer content of DraI sites or UV-B-induced mutations in specific DNA fragments, suitable hybridisation probes were used, 18S rDNA and rbcL
genes in this case.
To date, the primary DNA sequences that have been used to infer higher order angiosperm phylogenies include the mitochondrial genes atpA, cox1, SSU, the chloroplast genes rbcL
psaA, psbB and atpB, and the nuclear small ribosomal subunit (18S).
Samples from gametophyte populations from southeastern Alaska, British Columbia and Washington show no variation in rbcL
or rps4-trnS sequence and are similar to sequences from Asian H.
Higher level relationships of Apiales (Apiaceae and Araliaceae) based on phylogenetic analysis of rbcL