Detection of EBV gene expression in Reed-Sternberg cells
of Hodgkin's disease.
This could be due to insufficient sampling, as Reed-Sternberg cells
only represent a small percentage of cells in lymph nodes, meaning that the initial granulomas were simply "sarcoid-like reactions" in the setting of already active malignancy.
Hodgkin lymphoma (HL) is characterized by the presence of neoplastic giant cells called Reed-Sternberg cells
. These cells release factors that induce the accumulation of reactive lymphocytes, macrophages, and granulocytes, which typically make up greater than 90% of the tumor cellularity.
Biopsies of the lesion were consistent with Hodgkin's Lymphoma (HL), with Reed-Sternberg cells
that stained positive by immunohistochemistry for CD15, CD30 and stained negative for CD45.
It showed nodular pattern of mixed cellularity type of Hodgkin lymphoma characterized by background of eosinophils, plasma cells and lymphocytes, some Reed-Sternberg cells
(classic or mononuclear forms) with prominent large eosinophilic nucleoli (positive for CD15, CD30 and weakly PAX5, negative for S100, CD1a, CD20 and CD45), accompanied by LCH with diffuse neoplastic proliferation of Langerhans cells in other part (figure 2).
The bone marrow biopsy showed infiltration by scattered Reed-Sternberg cells
and mononuclear Hodgkin cells in reactive background consisting of small lymphocytes, epithelioid histiocytes, and occasional neutrophils and eosinophils (Figure 3).
Analyzing primary Hodgkin and Reed-Sternberg cells
to capture the molecular and cellular pathogenesis of classical Hodgkin lymphoma.
Hodgkin Lymphoma (HL) cell lines, including Reed-Sternberg cells
, has been shown to express CCR5, and clonogenic growth of these cells were directly attributed to engagement by different CCR5 ligands.
Olsson et al., "Expression of CCL5/RANTES by Hodgkin and reed-sternberg cells
and its possible role in the recruitment of mast cells into lymphomatous tissue," International Journal of Cancer, vol.
T-cell receptor PCR showed the presence of a clonal cell population and bone marrow histology revealed infiltration by tumour cells with scattered atypical uninucleated cells and binucleated Reed-Sternberg cells
. Immunophenotypic analysis showed no overt evidence of a B-cell lymphoproliferative disorder.
The diagnosis of HD is based on the recognition of tumour giant cells (Reed-Sternberg cells
) surrounded by benign-appearing host inflammatory cells (Fig.
The Reed-Sternberg cells
are binucleated or multinucleated and must be present in order for the diagnosis of Hodgkin's disease to be made.