replica plating


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replica plating

[′rep·lə·kə ‚plād·iŋ]
(microbiology)
A method for the isolation of nutritional mutants in microorganisms; colonies are grown from a microorganism suspension previously exposed to a mutagenic agent, on a complete medium in a petri dish; a velour surface is used to transfer the impression of all these colonies to a petri dish containing a minimal medium; colonies that do not grow on the minimal medium are the mutants.
References in periodicals archive ?
Replica plating was done by using sterile toothpicks to transfer isolates from the master 8 x 8 MacConkey agar grid plates to the corresponding 8 x 8 inoculated MH agar plate grid.
Se realizaron ensayos de replica plating para cada aislamiento previamente seleccionado.