They also play a part in "sterilizing" replication forks
that have stalled by treatment with DNA-damaging agents.
Each DNA lesion is recognized by specific sensor proteins according to the cell cycle phase in which the cell is; during S phase, the protein FANCM identifies a replication fork
arrested by an ICL, MRN + BRCA1 sense DSBs; meanwhile, Ku70/Ku80 + 53BP1 can recognize DSBs across the entire interphase; RPA detects and covers ssDNA primarily during S phase.
These include: (i) Assuring that DNA replication stops when the converging replication forks
reach specific chromosomal termination sites (ter sites); [sup] (ii) unlinking of the two interlocked DNA circles (catenanes) that are generated by the replication termination step [Figure 2]b; [sup] (iii) resolving the chromosomal dimers that are generated by recombinational crossover [Figure 2]c.
Diffley, "Is the MCM2-7 complex the eukaryotic DNA replication fork
helicase?" Current Opinion in Genetics and Development, vol.
Jentsch, "PCNA, the maestro of the replication fork
," Cell, vol.
Rolfsmeier et al., "Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks
," Molecular and Cellular Biology, vol.