We will use these in vitro replication systems together with both established and novel chromatin assembly systems to understand: a) how chromatin influences replication origin
choice and timing, b) how nucleosomes on parental chromosomes are disrupted during replication and are distributed to daughter chromatids, and c) how chromatin states and gene expression patterns are re-established after passage of the replication fork.
The important feature of present invention is the presence of the a bacterial replication origin
, which allows large DNA insert capacity.
Kyoto, Japan) has patented a plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin
that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide.
Gene Therapy: REPori is the minimal DNA replication origin
of the mammalian chromosomes.
Centrum Voor Plantenveredelings-en (Wageningen, Netherlands) has patented phagemid vectors comprising i) a promoter operatively linked to a gene coding for a translational fusion between a peptide and a filamentous single strand DNA bacteriophage coat protein or a part thereof, which promoter is induced by expression of gene IV of a filamentous bacteriophage, ii) a replication origin
derived from a filamentous single strand DNA bacteriophage, and iii) a plasmid replication origin