In addition, mtDNA possesses two main non-coding regions involved in replication and transcription processes: the control region or D-loop and the light-strand replication origin
or OL (Clayton, 1992; Shadel and Clayton, 1997; Boore, 1999).
DasSarma, "Minimal replication origin
of the 200-kilobase Halobacterium plasmid pNRC100," Journal of Bacteriology, vol.
coli paradigm in that the replication origin
, oril, contains multiple DnaA boxes where DnaA binds to initiate DNA replication [14-16].
These mutant variants were defective in maintenance of minichromosome, a plasmid with a cloned centromere and replication origin
The important feature of present invention is the presence of the a bacterial replication origin
, which allows large DNA insert capacity.
(Kyoto, Japan) has patented a plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin
that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide.
Centrum Voor Plantenveredelings-en (Wageningen, Netherlands) has patented phagemid vectors comprising i) a promoter operatively linked to a gene coding for a translational fusion between a peptide and a filamentous single strand DNA bacteriophage coat protein or a part thereof, which promoter is induced by expression of gene IV of a filamentous bacteriophage, ii) a replication origin
derived from a filamentous single strand DNA bacteriophage, and iii) a plasmid replication origin
In the paper, the team explain how the novel and essential bacterial replication origin
element directs single-strand DNA binding by the master initiator protein DnaA to promote opening of DNA duplex.
We will use these in vitro replication systems together with both established and novel chromatin assembly systems to understand: a) how chromatin influences replication origin
choice and timing, b) how nucleosomes on parental chromosomes are disrupted during replication and are distributed to daughter chromatids, and c) how chromatin states and gene expression patterns are re-established after passage of the replication fork.
This process of DNA replication is performed by complex, multi-component protein machines known as replisomes, which are assembled onto DNA at chromosomal sites known as replication origins
. These machines unwind the DNA double helix to form "fork" structures, at which the copying occurs.
At replication origins
, DNA replication starts bi-directionally during S phase, although only one out of five origins (920%) is actually activated in a cell cycle (For a review see ref.
We have shown that in some organisms, the replication origins
aACAo genetic switches that control DNA replication aACAo are not only unnecessary but cells will actually grow faster when these origins are not present.