In the present s study, we screened a total of 200 pesticides using our reporter gene assay
systems for detecting two ER subtypes, ER[alpha] and ER[beta], and AR activities.
Reporter gene assay
for the quantification of the activity and neutralizing antibody response to TNF[alpha] antagonists.
PPRE, Attagene cis-PPRE reporter gene assay
; PPAR[gamma], Attagene and NCGC trans-PPAR[gamma] reporter gene assay
and NovaScreen[R] hPPAR[gamma] direct binding assay; GR, Attagene cis-GRE, trans-GR, and NCGC trans-GR reporter gene assay
, and NovaScreen[R] hGR direct binding assay; LXR, Attagene trans-LXR[alpha], trans-LXR[beta] and NCGC trans-LXR[beta] reporter gene assay
; LXRE, Attagene cis-LXRE reporter gene assay
; C/EBP, Attagene cis-C/EBP reporter gene assay
; SREBP, Attagene cis-SREBP reporter gene assay
; RXR[alpha], Attagene and NCGC trans-RXR[alpha] reporter gene assay
Residues of the first 60 fractions representing the more polar part of the extract were dissolved in 270 [micro]l DMEM/F-12 and 100 [micro]l per well used in a promoter reporter gene assay
as described above using both murine (controlling a Renilla luciferase gene) and human (controlling a firefly luciferase gene (Prinzen et al.
neo--The luciferase reporter gene assay
can be used to evaluate whether xenoestrogens act via the classical nuclear hormone pathway (Joyeux et al.
We then examined the inhibitory potencies of the isolates (1-4) on 1L-6/STAT3 signaling pathway by using a STAT3 luciferase reporter gene assay
in HepG2 cells.
Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay
in the human T47D breast cancer cell line.
This study established a paclitaxel-resistant human breast cancer cell line (MCF-7/PTX) and applied the dual-luciferase reporter gene assay
, MTT assay, flow cytometry, transfection assay, Western blotting and the quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the reversing effects of paeonol and its underlying mechanisms.
These may include culturing primary hepatocytes alone or with a co-cultured reporter gene assay
, culturing three-dimensional liver model inserts (which are currently not applicable to high throughput) into wells along with a co-cultured reporter gene assay
, or using metabolically competent cell lines [e.
We performed a luciferase reporter gene assay
to test whether EERP could enhance transcriptional activity of LXRs.
Selected compounds were retested in follow-up studies that included a mutated ARE reporter gene assay
(where true active compounds should be inactive in this assay).