The same results could be obtained by dual-luciferase reporter gene assay
Unexpectedly, the Y473D mutation significantly diminished the transactivity of Chi as well as Ros and Pio in reporter gene assay
(Figures 3(d)-3(f)), despite no hydrogen bond between Chi and PPAR[gamma] at this region, which is different from SR1664, a known partial PPAR[gamma] agonist, showing no interaction with Y473 (Supplementary Figure 4) .
Thus, the use of this technique has revealed a close relationship between ADCC activity and infliximab levels, determined using a specific ELISA and infliximab activity determined using a TNF[alpha] responsive reporter gene assay
 in archival serum samples from patients with Crohn's disease.
For the reporter gene assays
, the treatment was regarded as a fixed factor and the day as a random factor.
 Nonstandard abbreviations: TNF, tumor necrosis factor; RA, rheumatoid arthritis; IBD, inflammatory bowel disease; CD, Crohn disease; ADA, antidrug antibody; mAb, monoclonal antibody; CHO, Chinese hamster ovary; CDR, complementarity determining region; PEG, polyethylene glycol; TNF-R2, TNF type 2 receptor; FDA, US Food and Drug Administration; FcRn, neonatal Fc receptor; HMSA, homogenous mobility shift assay; RGA, reporter gene assay
PPRE, Attagene cis-PPRE reporter gene assay
; PPAR[gamma], Attagene and NCGC trans-PPAR[gamma] reporter gene assay
and NovaScreen[R] hPPAR[gamma] direct binding assay; GR, Attagene cis-GRE, trans-GR, and NCGC trans-GR reporter gene assay
, and NovaScreen[R] hGR direct binding assay; LXR, Attagene trans-LXR[alpha], trans-LXR[beta] and NCGC trans-LXR[beta] reporter gene assay
; LXRE, Attagene cis-LXRE reporter gene assay
; C/EBP, Attagene cis-C/EBP reporter gene assay
; SREBP, Attagene cis-SREBP reporter gene assay
; RXR[alpha], Attagene and NCGC trans-RXR[alpha] reporter gene assay
Our study showed the transcription regulation relationship between Ngn3 and Sohlh1 by ChIP and luciferase reporter gene assay
for the first time, and proved that NGN3 protein may regulate spermatogenesis by forming complex with SOHLH1.Since Kaucher et al.
Reporter gene assays
(Figures 3(a) and 3(b)) showed that the lycopene-induced enhancement of cytokine-stimulated gene expression involved NF[kappa]B-binding sites.
The TRAC results were compared with a luciferase reporter gene assay
A reporter gene assay
of the cyclin D1 promoter was done under vitamin K2 treatment.
Further testing with luciferase reporter gene assay
revealed that each compound significantly activated the estrogen reaponse element reaulting in reporter gene expression (P = 0.0001).
The qHTS ARE beta lactamase reporter gene assay
(ARE-b/a) can detect compounds that activate the ARE pathway and induce oxidative stress (Attene-Ramos et al.