RP22-78A19, -44B20, -362C 15, - 127H7, -548M4, -160014, and RP23-430G14, -174D7, and -113D13 were used for all other experiments in this study, including PCR amplifications, sequencing, restriction mapping, and Southern blotting.
Predicted restriction enzyme cut sites in the database sequences were compared with actual restriction mapping fragment sizes and with the data from Southern blotting to determine the order of sequence contigs, An independent sequence assembly for this region obtained from Celera Genomics (GA_x6K02T2PU9B, as of 1 June 2002) was also used to fill in some gaps.
The mouse Cyp2a-t cluster spans about 1.4 Mb, as measured by restriction mapping. It is delimited by the Egln2 gene on the telomeric side and the Axl gene on the centromeric side (Figure 1).
RESTRICTION MAPPING OF DELETION BREAKPOINTS AND SEQUENCE ANALYSIS
To define the deleted portions including the deletion breakpoint, we performed A[upsilon]aII restriction mapping. As shown in Fig.
Two different large deletions were detected in four families by long-distance PCR and precisely characterized by restriction mapping and sequencing.