The tissue blocks were prepared, stained with Haematoxylin and Eosin stains, and special stains like PAS, Van-Gieson and reticulin
. IHC studies were done for relevant tumours.
The fourth patient had a one-grade improvement in the bone marrow reticulin
All liver biopsies were fixed in 10% formalin and routinely stained with hematoxylin and eosin, reticulin
, Masson trichrome, Periodic Acid-Schiff, and iron stain.
Formalin-fixed, paraffin-embedded bone marrow sections of primary myelofibrosis (PMF), acute monocytic leukemia (AML) with reticulin
myelofibrosis, multiple myeloma, chronic lymphocytic leukemia, and idiopathic thrombocytopenic purpura (ITP) were obtained from the Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin, China.
The final outcome is related to the size of angiomyoma, smaller than 5 cm, sparse mitotic activity in the histopathologic sample, abundant cytoplasm, prominent reticulin
fibers, and lack of invasion (2).
Portions of the liver were fixed with paraformaldehyde (0.1 mol/L, pH 7.4), paraffin-embedded, sectioned at 4 to 6 [micro]m and stained with commercial kits of reticulin
stain (D032, NJJCBIO, Nanjing, China) and Masson stain (D026, NJJCBIO, China) for microscopic examination after fixation for at least 2 days.
It has hyperplastic capillaries surrounding larger vessels in the central core of stem villi with increased numbers of loose, poorly cohesive lattices of perivascular bundles of reticulin
fibers and circumferential layers of pericytes.
The glial component resembles GBM and is richly positive for glial fibrillary acidic protein (GFAP), while the sarcomatous component resembles fibrosarcoma, but may also resemble osteosarcoma, chondrosarcoma, angiosarcoma,or rhabdomyosarcoma,5,6 and is rich in reticulin
and poor in GFAP expression.
Bone marrow biopsy revealed fibrosis with positive reticulin
stain [Figure 1]d.
Special histochemical stains such as Masson's trichrome were used to outline the basic framework of the tumor while reticulin
stain was used to outline collagen bundles, perivascular tumors, and to differentiate them from aggregates of epithelial tumors.
The nuclei are dark and lobulated and there is vacuolated glassy cytoplasm with the eosinophilic granular cell bodies and reticulin
fibers in the background.
Caption: Figure 3: (Reticulin
, 100x) hepatic parenchyma exhibiting subtle nodularity without significant fibrosis.