Stains for reticulin
and amyloid (Congo red stain) and immunohistochemistry for Tamm-Horsfall protein were negative.
In control as well as laser treated calves, reticulin
fibers were found more in number on 10th day interval and gradually decreased at 30th day interval.
Photomicrographs of vascularization evidenced by reticulin
staining in the zona compacta (SNc) of a control monkey (A) and a Modeling Parkinson's Disease in Primates (MPTP)-treated monkey (B)
Sections prepared by the silver impregnation technique showed pericellular deposition of reticulin
Sections were prepared and stains used included haematoxylin and eosin (HandE) Periodic acid Schiff (PAS) Methenamine silver (MS) and reticulin
The resulting biopsy specimens were embedded in paraffin and stained with hematoxylin/eosin (H&E), reticulin
, and Masson's trichrome for histological examination.
The bone marrow aspirate smears were stained by Geimsa stain while biopsy sections were stained by haemotoxyllin, eosin and reticulin
Collagen is the connective tissue contributing to less tender meat, as elastin and reticulin
are found in much smaller amounts and do not appear to contribute to unfavorable tenderness (Bailey, 1972).
Some lesions were found in adult connective tissue, formed by mature collagen scar fibers (type I collagen) and few inflammatory cells, whereas other lesions had reticulin
fibers (type III collagen) with numerous inflammatory cells.
Proteases released from eosinophils (collagenase, peroxidase, acid phosphatase and major B protein) is responsible for fragmentation of reticulin
fibers in the arterial wall.
Three to five micrometer thick paraffin sections were cut, and stained with hematoxylin-eosin and special stain reticulin
for examination under the light microscope.
Slides of the peripheral blood and bone marrow aspirates were stained using Romanowsky and May-Grunwald Giemsa (MGG) stains respectively; the trephine biopsy was decalcified, fixed, embedded in wax, cut, trimmed and stained using haematoxylin and eosin (H&E), MGG and reticulin