Data noted age at presentation, gender, medical history, physical examination, blood and bone marrow investigations such as, haemoglobin levels, blood cell count myeloperoxidase activity, periodic acid-Schiff and reticulin
staining as well as final diagnosis.
Focal and Diffuse fibrosis was seen in 20 (87%) cases, ranging from grade I to III by reticulin
staining and unremarkable in 3 (13%) cases.
September 2016: Bone marrow biopsy comments: Chronic lymphocytic leukemia (10% involvement); JAK2 mutation positive, marked reticulin
fibrosis, consistent with myeloproliferative neoplasm, such as primary myelofibrosis.
Sections were also stained with reticulin
and Masson's trichrome stain.
In addition to reticulin
stain, a number of immunomarkers have been shown to be of value in aiding in the distinction (Table 5).
On the same occasion, liver tissue samples were also taken, fixed with 10% formaldehyde, stained using hematoxylin and eosin (HE), periodic acid-Schiff and reticulin
stain protocols and analyzed with light microscopy.
Myxoma is defined as "a tumor composed of sometimes spindle-shaped cells set in a myxoid stroma containing mucopolysaccharide, through which course very delicate reticulin
fibers in various directions and which is locally invasive.
13) Grade 1 Loose connective tissue, cell-rich, old and new fibrin, fine reticulin
fibers 2 Connective tissue with cells and capillaries, few collagen fibers 3 Connective tissue more firm, fewer cells, more vessels, few elastic and smooth muscle fibers 4 Old firm granulation tissue, cell-poor, serosal layers hardly distinguishable TABLE 3.
Histologically, PXA has lipidized pleomorphic giant cells, fascicles of spindled cells in a reticulin
rich stroma, eosinophilic granular bodies (EGBs) and a lymphocytic infiltrate.
Porto-portal fibrosis was positive by trichrome/ reticulin
Hematoxylin and eosin stained sections were examined and few special stains such as PAS and reticulin
were performed wherever required.
Stain Solution Kit Histopathology (Ready to use)