reverse genetics


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reverse genetics

[ri¦vərs jə′ned·iks]
(cell and molecular biology)
An experimental method in which information from cloned deoxyribonucleic acid (DNA) or protein sequences is used to find or to produce mutations that help identify the function of a gene or protein (in contrast to classical genetics in which a known function or trait is traced back to a particular gene.
References in periodicals archive ?
it seeks to develop, with the help of an optimized system of molecular, systemic and systemic biology and reverse genetics, a whole new generation of whole-cell pertussis vaccine with markedly improved utility properties on the european and global markets.
These illustrations provide information for both forward (phenotype to genotype) as well as reverse genetics (genotype to phenotype) approaches.
This year's review of diseases of plants--mostly crops--contains 25 articles on such topics as Cladosporium fulvum effectors: weapons in the arms race with tomato, quarantine regulations and the impact of modern detection methods, the evolution and adaptation of wild emmer wheat populations to biotic and abiotic stresses, a multiscale approach to plant disease using the meta-community concept, developments in plant negative-strand RNA virus reverse genetics, and DNA methylation and demethylation in plant immunity.
Significant advances in basic biology, immunology, and vaccinology have led to a better understanding of the virus, and new tools such as reverse genetics make "it possible to make any live virus you want as long as you know what you want," he added.
Now, in a recent study, Yu used reverse genetics technology, which allowed him to generate new vaccines by inserting a gene from the ILTV virus into the NDV LaSota strain.
The simplified workflow for this powerful reverse genetics method can generate functional knockouts in one to two weeks versus the current one month timescale.
Since three years ago, we have begun our research on reverse genetics technology, the result of which has been the obtaining of this advanced technology to make various kinds of flu viruses," said Head of the vaccines division at Pars Inc.
Scientists first documented the cancer-fighting properties of Newcastle disease virus in the 1950s, but it is only with recent advances in reverse genetics technology that they have turned to the genetically engineered virus as a possible treatment.
Reverse genetics of RNA viruses; applications and perspectives.
By using site-directed mutagenesis and reverse genetics as described (9), we generated recombinant viruses with NA from the pandemic (H1N1) 2009 virus A/Auckland/1/2009 and the remaining genes from A/PR/8/34.
compile 12 articles by researchers in virology, microbiology, infectious diseases, immunology, and related fields in North America, Europe, New Zealand, and Australia who detail current research findings on caliciviruses and related diseases, including norovirus epidemiology, calicivirus contamination of the environment, genome organization and recombination, proteolytic cleavage and viral proteins, viral protein structures, virus-host interactions, reverse genetics and replicon systems, feline calicivirus, murine noroviruses, and lagoviruses.
A technique called reverse genetics was used to create a hybrid between the H1N1 swine flu virus and a tested laboratory strain.