The OTUs 02, 05, 08, 09, 10, 12 (respectively assigned to the genera Vogesella, Acidovorax, Shinella, Aeromonas and Rhizobium) were detected before the fifth day of degradation, while the OTUs 03 (unidentified Alphaproteobacteria), 13 (unidentified Rhodospirillales
), 07 (possibly genus Microvirga), 04 (assigned to the order Legionellales) and 11 (assigned to the family Sphingomonadaceae, possibly Novosphingobium) were found in the 9th and 15th days.
With regard to the Proteobacteria phylum, the three species that disappeared due to the oral sensitization procedure belonged to either the Alphaproteobacteria class (Rhodospirillales
order), the Deltaproteobacteria class (Spirobacillales order), or the Gammaproteobacteria class (Vibrionales order).
Our filtering approach recovered infections with Wolbachia (Alphaproteobacteria: Rickettsiales), Spiroplasma (Firmicutes: Entomoplasmatales), and Asaia (Alpha proteobacteria: Rhodospirillales).
We inferred a maximum likelihood phylogeny of these OTUs, their closest GenBank relatives, and other endosymbiotic Rhodospirillales bacteria from GenBank.
However, the symbiotic relationships of ants with Rhodospirillales have rarely been observed.
The phylogenetic history of ant-associated Rhodospirillales does not show host specificity and suggests likely acquisition from the environment (Figure 3).
The NGS probe revealed the presence of a long string of genera that don't appear in my college notes: Proteobac-teria, Alphapmteobacteria, Rhodospirillales
, Acetohacteraceae (OK, that's no surprise), Gluconohacter, Zymobacter (previously only found in palm sap), Oceanospirillales, and Dyellw spp.
isolates Actinomycetales 294 Bacillales 61 Pseudomonadales 56 Flavobacteriales 41 Burkholderiales 39 Lactobacillales 38 Enterobacteriales 33 Neisseriales 15 Pasteurellales 14 Rhizobiales 14 Clostridiales 10 Cardiobacteriales 9 Sphingomonadales 8 Caulobacterales 7 Rhodospirillales
7 Xanthomonadales 7 Fusobacteriales 6 Bacteroidales 5 Sphingobacteriales 4 Rhodocyclales 2 Desulfovibrionales 1 Micrococcineae 1 Rhodobacterales 1 Table 2.