SDS-PAGE


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SDS-PAGE

[¦es¦dē¦es′pāj]
(cell and molecular biology)
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
SDS-PAGE.--El SDS-PAGE de la fraccion albumina en condiciones reductoras mostro proteinas entre 6.5 a 45 kDa (Fig.
Protein profile analysis was performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method (Laemmli, 1970).
Analysis of allergenic proteins by Sodium Dodecyl Sulfate Polyacrylamide Gel: Allergenic proteins of different molecular weight were determined by using 12% SDS-PAGE. Stacking and resolving gels were prepared and poured immediately into the assembled gel casting apparatus and allowed it to polymerize for 40 minutes.
The C426 avimer recombinant protein was expressed under IPTG induction and detected using SDS-PAGE (according to band abundance the concentration of protein is predicted to be 5 [mu]g/[mu]l) and western blotting.
In order to have more information about the influence of thermal treatment on peanut and hazelnut proteins, raw and roasted extracts were separated by using SDS-PAGE and the corresponding protein profiles compared.
coli isolates obtained by SDS-PAGE were inspected visually and compared among samples.
Samples were actively rehydrated and put onto 7-cm pH 3-10 IPG strips (Amersham Biosciences) using Protean IEF cell (Bio-Rad) isoelectric focusing and then separated using SDS-PAGE. 2-DE was performed in triplicate for crude antigen of EgCF under the same conditions for IgE immunoblotting, IgG1 immunoblotting, and Coomassie blue G-250 staining.
SDS-PAGE results showed that both the supernatant and precipitate contained the target protein, but the supernatant had a higher amount of the target protein than the precipitate, indicating that the recombinant proteins mainly existed in soluble form (Figure 2(b)).
SDS-PAGE analysis for native bovine omasum laminae revealed presence of different proteins and after decellularization of it, the majority of the proteins were removed and only two protein bands (thick), one of 31 kDa for 24 h and another of 116 kDa and one protein band (thin) of 31 kDa at 48 h were present.
SDS-PAGE: The molecular mass of the purified enzyme was determined with the help of SDS-PAGE using 10% separating gel and the gel was stained with coomassie brilliant blue.The standard ladder was used to interpret the molecular weight of the enzyme.
Tubes containing fetuin peak were pooled together and compared to the original sample through SDS-PAGE electrophoresis showing that recovered fetuin presented the same electrophoretic pattern of the original sample (Figure 1B).