SDS-PAGE

(redirected from SDS-polyacrylamide gel electrophoresis)

SDS-PAGE

[¦es¦dē¦es′pāj]
(cell and molecular biology)
References in periodicals archive ?
The fifteen myofibrillar extracts of each species obtained by the denaturing or nondenaturing method were pooled and resolved by SDS-polyacrylamide gel electrophoresis in a gradient gel 8-18% [11].
SDS-polyacrylamide gel electrophoresis (PAGE) Broad Range or All Blue Precision Plus Protein (Bio-Rad Laboratories, Inc., USA) molecular weight standards were loaded at each end of the strip.
The homogeneity of the enzymes was checked on SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
The molecular mass of the enzyme was found to be 30 kDa in SDS-polyacrylamide gel electrophoresis. S.
The protein concentrations were determined by measuring the absorbance at 280nm, and proteins were analyzed by 12.5% SDS-polyacrylamide gel electrophoresis.
Only one peak was detected in F1, called F1-A (Figure 2a), and it produced a single band with a molecular mass of 50 kDa in SDS-polyacrylamide gel electrophoresis (Figure 3) in agreement with the molecular mass estimated for the gel filtration column.
(4), screens for proteinuria with few marker proteins (albumin and A1M) but uses additional marker proteins (IgG, transferrin, RBP, and ([[beta].sub.2]-microglobulin) to enable comparison with the SDS-polyacrylamide gel electrophoresis (PAGE) classification system introduced by Boesken et al.
Ten microliters of sample was separated by SDS-polyacrylamide gel electrophoresis (PAGE), and the protein bands were visualized by silver staining (SilverXpress Staining Kit, Invitrogen Corp., Carlsbad, CA).
Using a combination of gel filtration, anion-exchange chromatography, and reverse-phase high performance liquid chromatography, we isolated an acidic protein which shows a single chain composition, with a molecular mass of ~8 kDa, estimated by SDS-polyacrylamide gel electrophoresis, Its N-terminal sequence has high similarity to disintegrins isolated from different snake venoms, which are known to bind to cellular integrins such as the GPIlb/IIIa fibrinogen receptor on platelets.
Samples were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE).