The fifteen myofibrillar extracts of each species obtained by the denaturing or nondenaturing method were pooled and resolved by SDS-polyacrylamide gel electrophoresis
in a gradient gel 8-18% .
SDS-polyacrylamide gel electrophoresis
(PAGE) Broad Range or All Blue Precision Plus Protein (Bio-Rad Laboratories, Inc., USA) molecular weight standards were loaded at each end of the strip.
The homogeneity of the enzymes was checked on SDS-polyacrylamide gel electrophoresis
The molecular mass of the enzyme was found to be 30 kDa in SDS-polyacrylamide gel electrophoresis
The protein concentrations were determined by measuring the absorbance at 280nm, and proteins were analyzed by 12.5% SDS-polyacrylamide gel electrophoresis
SDS-Polyacrylamide Gel Electrophoresis
of Flagellin Protein.
Only one peak was detected in F1, called F1-A (Figure 2a), and it produced a single band with a molecular mass of 50 kDa in SDS-polyacrylamide gel electrophoresis
(Figure 3) in agreement with the molecular mass estimated for the gel filtration column.
Running of the SDS-Polyacrylamide Gel Electrophoresis
(4), screens for proteinuria with few marker proteins (albumin and A1M) but uses additional marker proteins (IgG, transferrin, RBP, and ([[beta].sub.2]-microglobulin) to enable comparison with the SDS-polyacrylamide gel electrophoresis
(PAGE) classification system introduced by Boesken et al.
Ten microliters of sample was separated by SDS-polyacrylamide gel electrophoresis
(PAGE), and the protein bands were visualized by silver staining (SilverXpress Staining Kit, Invitrogen Corp., Carlsbad, CA).
Using a combination of gel filtration, anion-exchange chromatography, and reverse-phase high performance liquid chromatography, we isolated an acidic protein which shows a single chain composition, with a molecular mass of ~8 kDa, estimated by SDS-polyacrylamide gel electrophoresis
, Its N-terminal sequence has high similarity to disintegrins isolated from different snake venoms, which are known to bind to cellular integrins such as the GPIlb/IIIa fibrinogen receptor on platelets.
Samples were analyzed by SDS-polyacrylamide gel electrophoresis