The spliceosome is a massive ribonucleoprotein complex comprising some small "U"s nuclear RNAs (snRNAs
) plus different specific proteins, these complexes being referred to as small nuclear ribonucleoproteins (snRNPs or SNURPs).
Usage of U7 snRNA
in gene therapy of hemoglobin C disorder: feasibility by gene ontology tool.
RNAsplicing is executed by a complex of proteins and small nuclearRNAs(snRNAs
) called the spliceosome.
A group of RNAs, small nuclear ribonucleic acids (snRNAs
), is required for eukaryotic cell life by virtue of the activity within a larger complex called the spliceosome.
We tested whether this "retro-evolution" approach could be a useful tool for exploring the origin and function of the small nuclear RNAs (snRNAs
) of the mammalian spliceosome.
was measured by the same method and used for normalization.
In this regard, noncoding RNAs (ncRNAs)  in the blood of cancer patients, comprising small nucleolar RNA (snoRNA), microRNA (miRNA), piwi-associated RNA, small Cajal body--specific RNA (scaRNA), and small nuclear RNA (snRNA
), have been suggested as novel biomarkers for noninvasive cancer diagnosis.
The cycle threshold was collected from each reaction, and the relative expression level of each miRNA to U6 snRNA
was evaluated using the equation 2-(CTmiRNA-CTU6snRNA), and the fold-change (log2-ratio) was used to show the differential expression pattern of miR-184 in different samples (Saleh et al.
EXOSOME PROTEINS MAY BE INVOLVED IN SNRNA
The relative quantification was calculated by comparative threshold (Ct) cycle method ([2.sup.-[DELTA][DELTA]Ct]) and normalized with U6 SnRNA
or GAPDH mRNA.
The ratio of each miRNA relative to the endogenous U6 snRNA
was calculated using the 2-aact method.
A cohort of five reference genes was simultaneously analysed for the normalization process (18S rRNA, RNU43, GAPDH, LAMIN A/C, and U6 snRNA
), and the optimum reference genes were identified using NormFinder algorithm .