SPECOL


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SPECOL

["SPECOL - A Computer Enquiry Language for the Non-Programmer", B.T. Smith, Computer J 11:121 (1968)].
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The following parameters were determined: dry matter--by drying at 105[degrees]C, total protein--by the Kjeldahl method, crude fat--by the Soxhlet method, crude ash--by incineration at 550[degrees]C, gross energy--using a KL-10 adiabatic bomb calorimeter, water-holding capacity--by the filter paper press method of Grau and Hamm (1953), color brightness--using a Specol spectrometer and an R45/O remission attachment, at a wavelength of 560 nm, pH--using a Radiometer PHM 22 pH-meter, fatty acid composition of intramuscular fat--by esterification (Peisker, 1964) followed by gas chromatography using a VARIAN CP-3800 chromatograph, at the following separation conditions: flame ionization detector FID, capillary column--length: 50 m, internal diameter: 0.
The absorbance of the solutions was recorded on the spectrophotometer SPECOL 10 at wave lengths 420-450 nm.
All enzymes were assayed spectrophotometrically by tracing the changes in absorbance at 27[degrees]C using UV-VIS SPECORD or SPECOL 11.
IN has been determined by the spectrophotometric method using a SPECOL 10 Karl-Zeiss Jena spectrophotometer, due to its ability to form a colored complex (absorption maximum at 520 nm) with vanillin in the presence of concentrated sulphuric acid.