Biopsy: Biopsy sample was inoculated into pre-solidified Sabouraud's agar
This yeast can grow in culture media devoid of lipids, such as Sabouraud's agar (1,11-13).
Among biochemical tests are catalase, [beta]-glucosidase, proliferation on Sabouraud's agar without lipid supplements, Tween and Cremophor-EL assimilation, phospholipase activity, and pigment production in media with tryptophan as only source of nitrogen.
Gram's staining and culturing on Sabouraud's agar
are recommended for diagnosis of candidiasis by Ritchie et al.
Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium (i.e., potato dextrose agar, malt extract agar, or Emmons' modification of Sabouraud's agar
Wood's lamp examination was carried out and the scrapings were cultured on Sabouraud's agar. A detailed dermatological examination was performed for evidence of fungal infection elsewhere in the body.
Skin scrapings were cultured on Sabouraud's agar to identify the infecting fungus, and appropriate treatment was prescribed.
Another portion was inoculated onblood agar chocolate agar Mac-Conkey agar Sabouraud's agar
brain heart infusion broth respectively in C-shaped streaks and cultured for the potential growth of bacteria fungi or acanthamoeba.
Biochemical tests were performed to determine the species: gelatin liquefaction, nitrate reduction, Loffler's serum slope, cyclohexamide agar, Sabouraud's agar, casein/tyrosine hydrolysis, in the temperature range 37 to 45 degrees centigrade.
Cladosporium carrionii, was isolated from culture on Sabouraud's agar. Prompt therapeutic response was seen to oral ketoconazole.
All were cultured for bacteria on sheep blood, MacConkey, and chocolate agar plates, and for fungi on Sabouraud's agar
. If requested by the submitting physician, specimens were forwarded to the nearby Centres for Disease Control and Prevention (CDC)-affiliated National Tuberculosis Laboratory for acid-fast bacillus (AFB) smear examination only.
Plain Sabouraud's agar
is needed to detect nondermatophyte fungi, such as Scytalidium, and Fusarium, that cause onychomycosis.