Their studies showed that one of these chemicals, sarcosine
, helps prostate cancer cells invade surrounding tissue.
We previously observed strong positive correlations between urine betaine, dimethylglycine, and sarcosine
among patients with coronary heart disease and found that high urine betaine was associated with new-onset diabetes during follow-up for approximately 3 years (11).
Bettocchi et al., "Serum sarcosine
is a risk factor for progression and survival in patients with metastatic castration-resistant prostate cancer," Future Oncology, vol.
Disks were placed in lysis buffer (6mM Tris-HCl, pH 8, 1 M NaCl, 100 mM EDTA, 0.5% Brij-58, 0.2% Na deoxycholate, and 0.5% N-lauroyl sarcosine
) with additional RNase (20 [micro]ag/mL) and lysozyme (1 mg/mL) and incubated for 4 h at 37[degrees]C.
The cells-containing agarose blocks were chilled at 4[degrees]C for 10 min and were submerged in EC-lysis solution (6 mM EDTA, pH 7.6, 0.5% Brij 58, 0.2% sodium deoxicholate, 0.5% sodium lauryl sarcosine
, 1 mg/ ml lysozyme, 10 mg/ml DNase-free RNase) and incubated at 37[degrees]C for 18 h.
Other prostate cancer-specific genes, includingPCA3 (prostate cancer antigen 3) (6),  TMPRSS2-ERG (transmembrane protease, serine 2) (7), DNA methylation (8), ERG (v-ets erythroblastosis virus E26 oncogene homolog) (9), annexin A3, and sarcosine
, some of which have been proposed in combination (10, 11), have raised enthusiasm throughout the scientific community.
The gel plug was suspended in Lysis II solution (1 mg/mL lysozyme, 0.5% Brij 35, 0.2% sodium deoxycholate, and 0.5% sodium lauryl sarcosine
in Tris-EDTA buffer) for 1 hour at 37 [degrees] C, replaced with ESP buffer (0.25 M EDTA, pH 8.0, 1% sodium lauryl sarcosine
, 0.5 mg/mL Proteinase K), and incubated at 50 [degrees] C for 2 hours.
Several bacterial strains were isolated that were able to convert glyphosate to sarcosine
and inorganic phosphate under phosphorus limiting conditions but such ability was lost when other P sources were made available (Dekker and Duke, 1995).
Folate (5-methyltetrahydrofolate), riboflavin, flavin mononucleotide (FMN),  FAD, pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxic acid, free choline, betaine, dimethylglycine, creatinine, methionine sulfoxide, arginine, asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA), tryptophan, kynurenine, kynurenic acid, anthranilic acid, 3-hydroxykynurenine, xanthurenic acid, 3-hydroxyanthranilic acid, and formiminoglutamic acid, were determined by liquid chromatography--tandem mass spectrometry (14-17), and methylmalonic acid, total homocysteine, total cysteine, methionine, serine, glycine, cystathionine, and sarcosine
by gas chromatography-tandem mass spectrometry (15, 18).
* Struktol Company, a leading producer of specialty additives for the rubber and plastics industry, has now entered the personal care market with its line of Perlastan sarcosine
Plugs were transferred to 2-mL round-bottom microcentrifuge tubes containing 1.5 mL of the previously described EDTA/ Sarcosine
buffer and 40 [micro]L of a 20-mg/mL solution of proteinase K.
The StatSensor (Nova Biomedical) handheld creatinine device uses capillary flow of whole blood into a test strip impregnated with creatinine amidohydrolase, creatine aminohydrolase, sarcosine
oxidase, and peroxidase which oxidize ferric-cyanide to ferrous-cyanide, producing a current ([e.sup.-]).