Injection into mouse cochlea through scala media by cochleostomy maximizes the efficiency as it allows virus to have access to many cochlear cell types.
Lang, "Adeno-associated virus-mediated gene delivery into the scala media of the normal and deafened adult mouse ear," Gene Therapy, vol.
In the scala media
, the target site where the OC lies, transplanted mIESCs were found in the lateral wall, basilar membrane, spiral limbus, and endolymphatic space (Figure 3).
The three fluid spaces (scala media
, scala tympani and scala vestibuli) are separated by the two membranes (basilar membrane and Reissners membrane).
The real mammalian cochlea is compound from three fluid spaces (scala media
, scala tympani and scala vestibuli) which are mutually divided by two membranes (basilar membrane and Reissner's membrane).