Schiff's reagent

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Schiff's reagent

[′shifs rē‚ā·jənt]
(analytical chemistry)
An aqueous solution of rosaniline and sulfurous acid; used in the Schiff test.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Subsequently, we fractionated the protein sample using SDS-PAGE and found two major groups of bands of around 30 kDa and smaller than 21 kDa by CBB staining and of around 150 and 30 kDa by Schiff reagent staining (Fig.
Periodic acid Schiff reaction (PAS).--Carbohydrate and glycoprotein deposits in sections of spider abdomens were localized by hydrolysis for 10 min in 0.5% periodic acid in distilled water, followed by a 10 min wash in running tap water and 15 min of staining in freshly prepared 1% Schiff reagent. After three 5 min rinses in sulfite water, another 10 min wash in running tap water and dehydration through absolute ethanol, preparations were air-dried and mounted in oil or resin.
The sections were coloured by Gomori's trichrome (1950), periodic acid-Schiff (PAS) (McManus, 1948; Bancroft & Cook), Alcian Blue Ph 2.5 and Ph1.0 (Pearse, 1985; Bancroft & Cook), Acetylation + PAS (Pearse), acetylation + deacetylation + PAS (Pearse), acid hydrolysis + PAS (Quintarelli, 1961), periodic acid + phenylhydrazine + Schiff reagent (Bancroft & Cook), periodic acid + thiosemicarbazide + Schiff reagent (Pearse; Bancroft & Cook) and observed in an Olympus BH-2 optical microscope.