Periodic acid Schiff reaction (PAS).--Carbohydrate and glycoprotein deposits in sections of spider abdomens were localized by hydrolysis for 10 min in 0.5% periodic acid in distilled water, followed by a 10 min wash in running tap water and 15 min of staining in freshly prepared 1% Schiff reagent
. After three 5 min rinses in sulfite water, another 10 min wash in running tap water and dehydration through absolute ethanol, preparations were air-dried and mounted in oil or resin.
The sections were coloured by Gomori's trichrome (1950), periodic acid-Schiff (PAS) (McManus, 1948; Bancroft & Cook), Alcian Blue Ph 2.5 and Ph1.0 (Pearse, 1985; Bancroft & Cook), Acetylation + PAS (Pearse), acetylation + deacetylation + PAS (Pearse), acid hydrolysis + PAS (Quintarelli, 1961), periodic acid + phenylhydrazine + Schiff reagent
(Bancroft & Cook), periodic acid + thiosemicarbazide + Schiff reagent
(Pearse; Bancroft & Cook) and observed in an Olympus BH-2 optical microscope.