sedimentation equilibrium


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sedimentation equilibrium

[‚sed·ə·mən′tā·shən ‚ē·kwə‚lib·rē·əm]
(analytical chemistry)
The equilibrium between the forward movement of a sample's liquid-sediment boundary and reverse diffusion during centrifugation; used in molecular-weight determinations.
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Sedimentation equilibrium experiments were performed on the cryo [kappa]-light chain protein that had been preequilibrated in the no-salt buffer and run at 6000 and 12 000 rpm at 20[degrees]C (analysis could not be performed at 4[degrees]C because of protein precipitation at higher concentrations).
It is possible that there are both salt- and temperature-dependent changes at 20[degrees]C and that only the salt-dependent changes in polymerization are detected by either sedimentation equilibrium or by the apparent [M.
3], for gastrula and pluteus, respectively (values were obtained by sedimentation equilibrium experiments; data not shown), and [[eta].
Fukui and Asai (1980) reported that Triton-treated immobilized cells oriented mostly upwards at the sedimentation equilibrium in sucrose density gradient.
Sedimentation equilibrium ultracentrifugation was used to assess the oligomeric state of synthetic NT-proBNP.
Structural assignment (a) Experimental 2[degrees] 3[degrees] technique structure structure SE-HPLC NA (b) Extended, nonglobular Sedimentation equilibrium ultracentrifugation NA NA CD Random coil, no helix NA Structural assignment (a) Experimental 4[degrees] Coiled-coil technique structure SE-HPLC NA NA Sedimentation equilibrium ultracentrifugation Monomer, not trimer No CD NA No (a) 2[degrees] structure refers to helix, [beta]-sheet, or random coil content; 3[degrees] structure refers to the overall three-dimensional shape of the molecule; and 4[degrees] structure refers to the putative state of association of individual molecules.
The sedimentation equilibrium analysis of the serum sample, performed in the presence or absence of DTT, revealed a molecular mass of ~128 kDa (Table 2).