Seliwanoff's test

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Seliwanoff's test

[sə′liv·ə‚nȯfs ‚test]
(analytical chemistry)
A color test helpful in the identification of ketoses, which develop a red color with resorcinol in hydrochloric acid.
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Prueba Extracto Et-OH CN Et-OH CL AA Ninhidrina - - Antrona + + Molish + Benedict +++ +++ Carbohidratos Barfoed (reductores) ++ + Seliwanoff + + Polisacaridos Lugol + + Polifenoles Foling +++ +++ Taninos Cloruro Ferrico Negro Verde Saponinas Cromatografia - + Cumarinas Cromatografia + + Prueba Extracto Et-OH SL Et-OH SN AA Ninhidrina + + Antrona + + Molish + + Benedict ++ ++ Carbohidratos Barfoed (reductores) + + Seliwanoff + + Polisacaridos Lugol + - Polifenoles Foling +++ - Taninos Cloruro Ferrico Verde - Saponinas Cromatografia + - Cumarinas Cromatografia - - Donde: (-) ausencia del metabolito, (+) presencia concentraciones bajas, (++) y (+++) presencia a una mayor concentracion del metabolito evaluado.
Fructosa: se determino mediante la prueba de Seliwanoff, para lo cual empleamos resorcinol al 0,5% y HCL concentrado y se expreso en [micron]mol/eyaculado (32, 33).
Fructosa: se determino mediante la prueba de Seliwanoff, para lo cual empleamos resorcinol al 0,5% con HCl concentrado y se expreso en [micron]mol/ eyaculado con un limite de referencia inferior (L.
Metabolic screening was initially normal; although, a second exam revealed positive Benedict, Seliwanoff, and nitrosonaphthol tests.
No data on hypoglycemia are presented, the Beneditt and Seliwanoff tests werepositive, while testsformucopolysaccharidosis resulted negative.
Up to about fifty years ago, the correct answer would have been to apply the Seliwanoff test.
D-Glucose was determinated using the glucose oxidase assay (FLEMING & PEGLER, 1963) and D-fructose was quantified as a keto-sugar using Seliwanoff reaction (BACON & BELL, 1948).
018 ** obtained by Seliwanoff assay; * obtained by glucose oxidase assay.
ANALYSIS OF TOTAL SUGAR IN THE HYDROLYZATE FROM SAWDUST WITH SELIWANOFF REAGENT
Therefore we use the Seliwanoff reagent for determining of actual concentration of total sugar in the hydrolyzate.