(8-12) The following features, however, define maturation-delayed germ cells, with the latter 2 serving to distinguish them from GCNIS: (1) expression of OCT3/4 beyond 6 months of age (Figure 1, C); (2) morphologic features of primordial germ cells/gonocytes (Figure 1, B); (3) central or parabasal position in the seminiferous tubules
; and (4) absence of such cells at the tubular basement membrane (ie, in the spermatogonial niche).
Estimation of seminiferous tubules
length: The length density of the tubules was calculated using an unbiased counting frame (25) at the final magnification of 148x using the following formula:
Diameter of seminiferous tubule
was expressed as mean +- standard deviation and the significant difference was determined using one way analysis of variance (ANOVA) followed by post Hoc Tukey test.
Group 3, Group 4 & Group 5 shows seminiferous tubules
with few spermatozoa and interstitial space with interstitial cells (Leydig cells) shows presence of few sperm cell within the lumen of the seminiferous tubules
vascular interstitial cells due to vascular interstitial cell moving spermatozoa along the lumen of the tubules EGME Treatment (Fig.
In addition to the severe exfoliation of the degenerative and germinative epithelium, other alterations were observed, including a reduction in the number of spermatogonia and spermatocyte layers, accumulation of proteinaceous material in the lumen of the seminiferous tubules
and absence of epithelial cell stereocilia in the epididymis (Figure 2).
Percentage of seminiferous tubules
containing multinucleated giant cells
The results of the microscopic examination showed no clear difference between the densities of spermatogonia type A, primary spermatocytes at the pachytene stage, spermatids at the zygotene stage and spermatozoa in the seminiferous tubules
of vaccinated (Figure 2Ba) and control lambs (Figure 2Bb) at week 30 after the primary immunization.
Hence, seminiferous tubules
only contain spermatocytes at the end of the development of germinal cells.
Also previous studies have showed that ghrelin-ip cells were distributed in the seminiferous tubules
and interstitial tissues of the testes of human, mouse and sheep (David et al., 2005; Gaytan et al., 2004; Ilhan and Erdost, 2013).
Furthermore, the relative frequency of each stage was determined using 800 cross-sectional seminiferous tubules
(400 cross sections per animal) referring to the staging of Almeida et al.
In the control and GnRHant groups (Figures 1(a) and 1(d)), the seminiferous tubules
had a normal, thick, and smooth germinal epithelium.
In a review, Parenti and Grier (2004) observed an evolutionary net on the teleosts testis morphology, through the organization of the seminiferous tubules
and the distribution of spermatogonia throughout the germinal epithelium.