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This is a quick method of sertoli cell culture, but one of important disadvantages of this method is that apart from sertoli cells, the other myeloid cells such as fibroblasts attached to the bottom of the petri dish.
The epithelium consists of Sertoli cells and scarce spermatogonia, condition that is observed throughout the whole tubul.
The movement of preleptotene/ leptotene spermatocytes across the blood-testis barrier (BTB), also known as the Sertoli cell barrier, during the seminiferous epithelial cycle is one of the most important cellular events taking place in the mammalian testis.
Transduction of Sertoli cells with adenoviral and lentiviral vectors carrying the mouse Steel gene has been found to restore spermatogenesis (30,35).
And increased content of testicular spermatozoa, improvement in the number of spermatocytes, it returned her natural layered arrangement inside Seminiferous tubule, as restore sertoli cells its contact with both other germ cells and spermatocytes, the leydig cells have been observed to increase the number and size, nuclei return to normal in terms of form and place (Figs 4,5,6 ) .
Sertoli cells are critical in sperm production, and it was shown that reduced testosterone levels are coupled with decreased expression of steroidogenic acute regulatory protein (StAR) and 3[beta]-hydroxysteroid dehydrogenase (3[beta]-HSD) [25, 26].
Oestrogen-induced bone marrow aplasia in a dog with a Sertoli cell tumour.
However, in these species germ cell development is clonal, since each spermatogonium is surrounded by a single Sertoli cell in the seminiferous lobules; and thus a group of clonal germ cells arising from a single spermatogonium develops synchronously within the cyst (Schulz et al.
The Differential Diagnosis of Sertoli Cell and Sertoli-Leydig Cell Tumors
Environmental toxicants perturb human Sertoli cell adhesive function via changes in F-actin organization mediated by actin regulatory proteins," Human Reproduction 29(6): 1279-1291.