Wolf spiders have four types of silk glands
: piriform, ampullate, aciniform and tubuliform (Richter 1970).
The stable protein concentration was maintained at pH between 5.0 and 6.5 that might be associated with the spinning process (decrease from 6.9 to 4.8 along the path) in the silk glands
Voight's team also examined gene transcripts from different orb-weaver silk glands
and in each case found transcripts belonging to more than one spidroin class, suggesting that these glands are not strictly specialized for producing one type of silk.
Another discovery made by the team was that of 649 genes that are likely not spidroins but were highly expressed in silk glands
of the orb-weaver.
The larvae silk glands
were removed and homogenized in 150 [micro]L of the extraction solution (2-mercaptoethanol 0.1% and 50 [micro]L of carbon tetrachloride).
Since the silk gland
forms the basic organ for cocoon formation and silk production (Krishnaswamy, 1978), probably the synthetic activities of the protein metabolism might have been accelerated through anabolic process by the hormonal treatment, (Bharathi et al., 1986).
The araneoids have six functional spinnerets and six different kinds of silk glands
each producing one to three types of protein (Kovoor 1987; Coddington 1989).
Aggregate silk gland
secretions of araneoid spiders.
"We already knew that honeybee silk fibres could be hand-drawn from the contents of the silk gland
so used this knowledge to hand-draw fibres from a sufficiently concentrated and viscous mixture of the recombinant silk proteins," she added.
"Numerous efforts have been made to express other invertebrate silks in transgenic systems but the complicated structure of the silk genes in other organisms means that producing silk outside silk glands
is very difficult.
The silkworm larvae from all the five instars, excreta leaves and silk glands
were used for AAS analysis.
With one longitudinal cut on the dorsal surface the larvae were cut open and their silk glands
were isolated in 9% NaCl.