Vass, Temperature and Concentration Dependence of Properties of Sodium Dodecyl Sulfate
Micelles Determined from Small-Angle Neutron Scattering Experiments, J.
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Using mass spectrometry analysis, and after elution of the captured populations in 4% boiling sodium dodecyl sulfate
, the researchers categorized the species in 20 different barley protein families and two maize proteins, which were the only ones to survive the brewing process.
The researchers deamidated two commercial SPIs using sodium dodecyl sulfate
(SDS), an anionic surfactant.
After hybridization, the slides were rinsed and then washed successively at room temperature with 2x SSC containing 1 g/L sodium dodecyl sulfate
After incubation, 10 [micro]L sodium dodecyl sulfate
(SDS) sample buffer and 7 [micro]L of reducing agent were added to 40 [micro]L of each mixture, and the proteins solubilized by boiling (10 min).
They used such techniques as sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE), western blotting techniques and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
We have previously shown that the addition of sodium dodecyl sulfate
(SDS) to the PRM reagent increases the interference, whereas the addition of sodium oxalate reduces the interference (6, 7).
Plasmid extraction was performed with several techniques, including lysis with sodium dodecyl sulfate
(10) and alkaline lysis conducted with a conventional method (10) or with the Nucleobond BAC100 system (Macherey-Nagel, Duren, Germany).
To do this, they used sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE) and a competitive inhibition Elisa, respectively.
2], cells were washed with PBS and lysed with sodium dodecyl sulfate
(SDS)--polyacrylamide gel Laemmli sample buffer.
The effect of sodium oxalate contrasts with the addition of sodium dodecyl sulfate
to the PRM reagent (7), which increases aminoglycoside interference (8).