Subsequently, to obtain single-copy insertion transgenic lines, Southern blot
analysis was performed.
As represented in Figure 4B and 4C, genomic DNA analyses by PCR and Southern blot
showed that all of the piglets were homozygotes with disruption in both alleles of [alpha]GT gene.
Total DNA from three randomly selected independent J7-F transformants containing pFJ1-M-3916, pFJ4-M-3916, and pFJ6-M-3916, respectively, was digested with Sau3AI, separated by 1% agarose gel electrophoresis, and subjected to Southern blot
analysis using a DIG-labeled 3916 pro probe.
For Southern blot
analysis of transgene copy number, approximately 15 [micro]g of RNase A-treated rice genomic DNA was digested with SpeI and separated in 1% agarose gel along with 1 kb plus DNA marker (Gene Ruler, Thermo Fisher Scientific, Walthan, MA) in 1x TBE buffer and transferred onto the Nylon-[N.sup.+] membrane (GE Healthcare Amersham).
(21,22) PLD4-knock-in ES cells were identified by Southern blot
analysis (Supplemental Fig.
In addition, Southern blot
analysis indicated that the mean difference of telomere length between first and last passage was 0.25 kb in DPSC, 0.1 kb in PAFSC and 0.32 kb in HFF which indicates that the mean telomere length in various passages of the samples showed insignificant changes.
As established by Pfister et al (10) and Ozden et al, (4) HPV subtypes 13 and 32 have been identified as the cause for FEH lesions through polymerase chain reaction (PCR) analysis and Southern blot
S1 nuclease pulsed-field gel electrophoresis and Southern blot
analysis were used to identify the sizes of [bla.sub.NDM-1]-carrying plasmids.
The presence of begomoviruses was also confirmed by Southern blot
analysis using cloned DNA-A probe of Cotton leaf curl virus.
Con el fin de detectar el elemento dTdic1 en el DNA genomico de clavel, se realizo Southern blot
utilizando el Kit Dig High Prime Labeling and Detection Starter Kit II de ROCHE[R], siguiendo el protocolo descrito en DIG Application Manual for Filter Hibridization de ROCHE[R].
Single and double crossover colonies were confirmed by PCR and southern blot
. Southern blot
was carried out using chromosomal DNA from the wild type parent and the deletion mutant strains, and the 'ECL direct nucleic acid labeling and detection system' kit (GE).
Southern, the Southern blot
remains a standard molecular biology technique for determining the arrangement of DNA sequences within a vector or genome (Southern, 1975).