to measure TRF length was performed for HinfI-digested genomic DNA by using [sup.32]P-labeled [(TTAGGG).sub.4] probe as described previously (11).
. The digested DNA was separated overnight at 25 V on a 0.8% agarose gel in Loening buffer (36 mmol/L Tris-Cl, 30 mmol/L Na[.sub.2]P[0.sub.4], 1 mmol/L NaZEDTA) and transferred in 0.4 mol/L NaOH for 1 h onto a positively charged Nylon membrane (Qiagen), using the Turboblotter Rapid Downward Transfer System (Schleicher & Schuell).
*In the application segment, nucleic acid accounted for the largest share in 2016, owing to the large application of DNA markers in PCR, sequencing, northern blotting, southern blotting
, and molecular cloning
PCR and Southern Blotting
Detection of Transformants
Integration of the MsHsp23 gene into the creeping bentgrass plants genome was further confirmed by Southern blotting
The DNA fragments were separated by gel electrophoresis for 24 hr at 70 V on a 0.8% agarose gel and transferred to a nylon membrane by Southern blotting
. The blotted DNA fragments were hybridized to a digoxigenin (DIG)-labeled probe specific for telomeric repeats and incubated with a DIG-specific antibody covalently coupled to alkaline phosphate.
TL was measured by Southern blotting
from DNA samples extracted from white blood cells.
Therefore, college molecular biology instructors frequently cover Southern blotting
, as well as the related, and humorously named, "Northern" blot, where RNA is detected in a similar fashion (Alwine et al., 1977).
slightly enhanced the sensitivity of the PCR, but visual inspection of gel-resolved PCR products from feces was nearly equally sufficient (Table and Figure).
Alternatively, more precise PCR-based sizing of the PM alleles and/or detection of FM alleles up to approximately 200 repeats by capillary electrophoresis (or up to approximately 1300 repeats by agarose gel electrophoresis), as well as the methylation status, are now possible (43, 44), although the performance of these PCR-based methods will need to be reviewed and compared with Southern blotting
, which is still the gold standard, before they can be adopted as diagnostic tools.
PCR and Southern blotting
positive rates were analyzed using Yate's corrected [chi square]-test of independence and p<0.05 was considered statistically significant (Snedecor and Cohran, 1980).
Chapter topics include the cell, protein synthesis, gametogenesis, the place of genetics in medicine, autosomal recessive inheritance, chromosome structural abnormalities, twin studies, immunogenetics, gene mapping, dysmorphology, southern blotting
, DNA profiling, an avoidance and prevention of disease.