Seminiferous tubules were labeled as type A, B, C according to the spermatogenetic activity variations between tubules; A: Tubule consisting of sertoli cells and spermatogonia, no spermatocytes or spermatids
; B: Tubule consisting of sertoli cells, spermatogonia and spermatocytes, no spermatids
; C: Tubule consisting of sertoli cells, spermatogonia, spermatocytes and spermatids
However, in the present study apoptosis was diffused in spermatogonia, spermatocytes, and spermatids
in seminiferous tubule in the group treated with 7 mg/kg dose level.
It is possible that endosulfan, by the creation of free radicals (ROS) and mutations in the testis tissue, especially spermatogonia, primary spermatocytes, spermatids
and spermatozoa sensitive cells can cause serious damage and loss of these cells.
The remarkable observation that infected natural hybrid individuals have a significantly higher frequency of abnormal spermatids
firmly supports this hypothesis.
Table 2 shows the reproductive organ weights, as well as sperm and spermatid
counts, sperm morphology, and steroid hormone levels.
During spermiogenesis, the spermatid
nucleus changes in shape and decreases in volume as the chromatin condenses.
The number of step-19 spermatids
was reduced by 87.
Group C showed partial reversal of these effects with presence of round and elongated spermatids
In addition, spermatogonia and spermatids
(round and elongated) are found to be specifically positive to phosphotyrosine antibody (Fig.
3A, B), where spermatogonia lays adjacent to the acinus wall and spermatocytes lay near the inner of the wall, followed by spermatids
and spermatozoa, located toward the center of the acinus.
The lumen of the seminiferous tubules is characterized by different stages of spermatids
and mature spermatozoa (Fig.
37a Spermatogonia 9,28 [+ or -] 1,62a 9,22 [+ or -] 1,64a type A Pachytene 48,61 [+ or -] 3,74a 49,03 [+ or -] 2,34a spermatocyte Round spermatid
147,11 [+ or -] 4,33a 148,24 [+ or -] 2,83a Parameter TB TC (a) Stages of spermatogenesis I-VI 46.