However, no significant difference was observed in the number of spermatogonia (A and B), spermatocyte, round and long spermatids
, and Sertoli cells between the experimental groups.
The control negative group (200x; H&E) has normal cyto-architecture, reveal interstitial tissues (IT) and seminiferous tubules (ST) with sustentacular cells and the normal developing germ cells: spermatogonia (Sp), spermatocytes I (SPI), spermatids
(Spt), spermatozoa (Spz) with flagellum.
This condition explains the elevated frequency of the I-VI stage of the spermatogenisis of the pubertal animals in this study, because it is in the stage that occurs the greatest presence of young and mature spermatids
Category 9 denotes many end-stage spermatids
, value 8 less than five spermatozoa and few late-stage spermatids
For these purposes, there are two methods to characterize stages of the seminiferous epithelium cycle: (1) based on tubular morphology and (2) based on development of the acrosomic system and nuclear morphology of developing spermatids
A) Primary spermatocytes (40X); B) Secondary spermatocytes (40X); C) Spermatids
(40X); D) Spermatozoa (40X); E) Spermatozeugmata (20X).
Homogenization-resistant testicular spermatids
(stage 19 of spermiogenesis) and sperm in the caput/corpus epididymis and cauda epididymis were assessed as described previously by Robb, Amann, and Killian (1978), with adaptations of Fernandes et al.
Some abundant spermatocytes, ripe oocytes with a large smaller spermatids
, and nucleus may be observed ripe spermatozoa with free in the lumen.
Testicular spermatogenesis comprises a precisely timed and synchronized development of several generations of germ cells involving spermatogonial mitosis (proliferative phase); spermatocyte in which genetic material is recombined and segregated (meiotic phase); morphological transformation of the undifferentiated spermatids
into highly specialized motile sperms (spermiogenic phase) .
(1) Abbreviations: Int, uncharacterized interstitial cells; Lc, Leydig cell; Sc, Sertoli cell; Spg, spermatogonia; Spc, spermatocyte; Spd, spermatid
; Spz, spermatozoa; E, epithelium.
"Actin binding proteins, spermatid
transport and spermiation," Seminars in Cell & Developmental Biology 30; 75-85.
Recent immunohistochemical studies in tissues from rat testes showed that MARCH7 is highly expressed in developing rat spermatids
, colocalizing with [beta]-actin.