Testicular cell number estimation: To estimate the total number of spermatogonia A (pale-staining nucleus with a fine "dusty" distribution of heterochromatin throughout the nucleus), B (with dense clumps of heterochromatin around the periphery of the nucleus), (24) Sertoli cells, spermatocytes
, round and long spermatids, and Leydig cells, a Nikon E200 light microscope (Tokyo, Japan) fitted with a 60x oil objective lens was applied at the final magnification of 1,640x.
The control negative group (200x; H&E) has normal cyto-architecture, reveal interstitial tissues (IT) and seminiferous tubules (ST) with sustentacular cells and the normal developing germ cells: spermatogonia (Sp), spermatocytes
I (SPI), spermatids (Spt), spermatozoa (Spz) with flagellum.
Score 5 denotes no spermatozoa or spermatids and many spermatocytes
; score 4 no spermatozoa or spermatids and few spermatocytes
Black perch < 90 mm SL did not have spermatozeugmata within the testicular tissues but all stages of spermatocyte
development were present (Fig.
In adult rats, Madhu, Sarkar, Biswas, Behera, and Patra (2011) observed reduction in seminiferous tubular areas, secondary spermatocytes
and nuclear diameter of Leydig and Sertoli cells after 45 days of exposure to 1 mg [kg.sup.-1] bw [day.sup.-1] of 3-MCPD.
abnormalities Primary rat spermatocytes
stained with an anti-SCP3 antibody were selected to quantify synaptonemal complex abnormalities by fluorescence photomicroscopy.
In a study, administration of 200 and 300 mg/kg to rats for 60 days showed a significant increase in the number of sperms, sertoli cells, and primary spermatocyte
compared to the control group.
Seminiferous tubules were labeled as type A, B, C according to the spermatogenetic activity variations between tubules; A: Tubule consisting of sertoli cells and spermatogonia, no spermatocytes
or spermatids; B: Tubule consisting of sertoli cells, spermatogonia and spermatocytes
, no spermatids; C: Tubule consisting of sertoli cells, spermatogonia, spermatocytes
Radiation-induced chromosome aberrations in mouse spermatocytes
Effects ofgamma rays on intact spermatocyte
cells, with complete cessation of the spermatogenesis process, was reported on peach fruit flies (Shehata et al.
After the meiotic division, a smaller secondary spermatocyte