For the isolation of aerobic sporulating bacteria
, the dilutions were heated in a water bath at 92[degrees]C for 15min before being inoculated onto nutrient agar plates.
Thus, a possible solution would be the development of a less costly process to obtain ureolytic sporulating bacteria
. It must be possible to select an ureolytic sporulating bacterial community starting from soil, wastewaters, activated sludge or any kind of material rich in active microbial activity.
Sporulating bacteria were avoided from WS3 by picking colonies that could survive at maximum temperature and chemical concentration.
Results not only indicated the decrease in bacterial population by 50 times at 37[degrees]C, but also revealed the presence of sporulating bacteria in soil, as spores can tolerate high degree of osmotic potential, temperature and irradiation.
Lactic acid bacteria are gram positive, catalase negative, non sporulating bacteria
that can ferment various carbohydrates to lactate.
Absence of the contaminating bacteria growth in medium-H confirms the absence of heterotrophic sporulating bacteria
and therefore such observation confirms the isolation of Thiobacillus thermosulfatus in pure form.
Soil samples were analysed by the plate count to determine the number of: oligotrophic bacteria, oligotrophic sporulating bacteria on 100-fold diluted nutrient bullion, and total copiotrophic and copiotrophic sporulating bacteria on concentrated nutrient bullion using the method of Onta and Hattori (1983).
The number of oligotrophic sporulating bacteria was increased in objects with petrol and main crop and in objects with diesel oil and aftercrop (tab.1).
The proliferation of oligotrophic sporulating bacteria and copiotrophic sporulating bacteria (only in series with diesel oil) during the vegetative growth decreased, whereas that of oligotrophic bacteria and copiotrophic bacteria increased, reaching the maximum during the oat harvest.
Although bentonite and calcium oxide increased the average number of oligotrophic and copiotrophic sporulating bacteria in soil, except oligotrophic bacteria in series with diesel oil and CaO application at the oat harvest.
Other difficulties can occur with classifying diseases caused by sporulating bacteria
(Clostridium and Bacillus): Their infective spores survive in the soil or in other substrata for very long periods, though they are usually produced after a vegetative growth in the abiotic environment, which can include animal carcasses.