(18)-(20) AU-rich elements (AREs) and stem-loop
structures in 3' UTRs are well-studied examples of cis-elements recognized by a set of RBPs for controlling the stability of target mRNAs (Fig.
IIIa and IIIc were conserved among PBMCs and plasma-derived IRESes, except for a single mutation in stem-loop
IIIc at position 234 detected only in PBMC-derived samples (Figure 1).
As shown in Figure 1(a), at temperatures below 45[degrees] C, fluorescence intensity does not change with increasing temperature, which indicates that molecular beacon can maintain a stable stem-loop
We used a stem-loop
RT-PCR method to quantify the expression levels of various miRNAs.
In oocytes, some related factors are coupled to the SLBP, which contains the only RNA binding region and interaction with stem-loop
The OL region located between tRNA-Asn and tRNA-Cys comprised 35 bp in length within the WANCY region (Supplementary Table S1) and predictably had the capacity to fold into a stable stem-loop
hairpin structure consisting of 13 bp in the stem and 11 bp in the loop.
In the present study, we investigated the miR profiling in a small group of Chinese GC patients by applying a high throughput miR sequencing method and then validated the results by performing stem-loop
qRT-PCR in vivo and in vitro.
Whether a stem-loop
hairpin present in secondary structure of the sequences is a common feature of miRNA precursors, the ESTs failed to meet this criterion are eliminated.
configurations can also be used to monitor global environmental changes such as changes in temperature by using a fluorophore-quencher pair on the ends of the strands.
HV-GcV2 (2,262 nt) shares the same stem-loop
motif and genomic structure with HV-GcV1, with the exception of the rep gene, which is coded by a single open reading frame.
Comparison of the secondary structures of our isolated aptamers and aptamer A10 obtained by protein SELEX showed similar stem-loop
structures which could be responsible for the recognition of PSMA on LNCap cell surface.