cell fractionation

(redirected from Subcellular fractions)

cell fractionation

[¦sel ‚frak·shə′nā·shən]
(cell and molecular biology)
A laboratory technique that uses differential centrifugation to separate the different components of the cell, resulting in nuclear, mitochondrial, microsomal, and soluble fractions.
References in periodicals archive ?
However, except for localization in the lysosome, GH is also localized in other organelles, including the mitochondria and Golgi apparatus (Lobie et al., 1994), suggesting that GH may function in subcellular fractions. Indeed, Strous indicated that the GH-GHR complex is internalized into the cytoplasm and subsequently transmits signals (van Kerkhof et al., 2000).
(b) Neutrophil subcellular fractions prepared as above were processed for immunoblot analysis of the cytosolic marker leukotriene A4 hydrolase (LTAH) or the nuclear marker histone H3 (HH3).
(b) Semiquantitative analysis of AR levels in the subcellular fractions after exposure of the cells to vehicle (basal) or testosterone for 30 min.
Isolation of Subcellular Fractions. Around 4g of liver tissue was homogenized in 8 mL of Buffer A (225 moles/L sucrose, 10mmol/L Tris-HCl, and 0.3mmol/L EDTA, pH 7.4; thereafter, the homogenate was centrifuged at 1,800 g 15 min at 4[degrees]C.
The protein levels of GAPDH in subcellular fractions were detected by Western blotting with beta-tubulin as control.
In connection with the above-stated, the main purpose of investigation was comparative evaluation of the effect of Decis on the levels of nucleic acids and protein in the subcellular fractions of the liver and intestinal mucosa in the experimental animals under the conditions of optimal and high temperature of air as well as correction of the levels of nucleic acids and protein by administration of the complex of bioactivators.
The part of animals were killed in 1, 2, 7, and 15 days after and the concentration of DNA, RNA and total protein were measured in the subcellular fractions of the liver and the intestinal mucosa.
disposable conical tubes via 8- and 14-place rotors ideal for pelleting cells, bacteria, subcellular fractions and small amounts of nucleic acids and viruses with more than double the throughput of a standard fixed-angle rotor and high g-forces up to 24,000 xg.
A sampling of topics: genome and proteome analysis of Chlamydia, Helicobacer pylori vaccine development based on combined subproteome analysis, web-accessible proteome databases for microbial research, continued proteomic analysis of Mycobacterium leprae subcellular fractions, the cell wall subproteome of Listeria monocytogenes, and induction of Mycobacterium avium proteins upon infection of human macrophages.
Comparison of selenium and mercury distribution in porcine tissues and their subcellular fractions studied by NAA and HG-AFS.
Samples of the resultant subcellular fractions were used for heat shock, phosphoprotein and chitin synthase studies.