A limitation of the flow injection approach is that sulfatase
substrates undergo nonenzymatic cleavage to desulfated products in the heated ESI source of the mass spectrometer, which interfere with the activity measurements.
Decreased specific activity of sulfatases
was observed only on the 30th day of DM.
Two pathways are important for the local E2 production in target tissues, namely, the "sulfatase
pathway," in which biological inactive steroid sulfates are the source for E2, and the "aromatase pathway," in which E2 is derived from androgenic precursors .
activity and expression in mammary myoepithelial cells.
The multiple sulfatase
deficiency gene encodes an essential and limiting factor for the activity of sulfatases
Like P, sulfate esters are cleaved by hydrolytic enzymes called sulfatases
to release S[O.sub.4.sup.2-].
For example, sulfatases
are being developed for use in enzyme replacement therapy.
An alternative explanation for the enhanced mineralisation of organic S with lower arylsulfatase activity in soils under forest is that the measurement of arylsulfatase activity does not reflect the overall real enzyme activity, since there are several types of sulfatase
enzyme present in soil (including arylsulfatases, alkylsulfatases, steroid sulfatases
, glucosulfatases, chondrosulfatases, and myrosulfatases) (Tabatabai and Bremner 1970).
MS/MS assays of the sulfatases
relevant to the LSDs MPS-II, -IIIA-D, -IVA, and-VI have been reported (68-72).
Biology and function of the reversible sulfation pathway catalysed by human sulfotransferases and sulfatases
. Chem Biol Interact 109:3-27.