A 0.1 mL volume of the supernatant was aliquoted into a test-tube and added to this was 1.9 mL of distilled water and 1 mL of 1% sulphanilamide
reagent (prepared in 1N HCl) followed by 1 mL of 0.02% N-1-naphthyl ethylene diamine dihydrochloride.
Skin treatment by microwave followed by solid pectin film loaded with sulphanilamide
and oleic acid result in permeation of all drug molecules that are released from film .
Sheehan, 'The story of sulphanilamide
', KT, February (1939), pp.64-65.
Nitrite nitrogen was determined using sulphanilamide
in an acid solution.
Briefly, conditioned cell culture media (100 [micro]L) were mixed with 100 [micro]L of the Griess reagent (1% sulphanilamide
and 0.1% N-[1-naphthyl]-ethylenediamine dihydrochloride in 5% phosphoric acid) for 10 min.
gonorrhoeae as the bacterium that causes gonorrhoea in 1879, it was not until 1937 that the first curative treatment came with the introduction of sulphanilamide
, an antibiotic of the sulfonamide group.
For Nitrogen dioxide determination by colorimetric method, one part distilled water; one part sulphanilamide
reagent (20g sulphanilamide
+ 50ml concentrated orthophosphoric acid diluted to 1000ml with distilled water) and one tenth part N-1-naphthyl-ethylene- diamine-dihydrochloride (NEDA) reagent (0.350g NEDA in 250ml distilled water) were added to each sample tube at the opposite end to the mesh collectors.
After 150 min, 0.5 ml of Griess reagent (equal volume of 1% sulphanilamide
in 5% ortho phosphoric acid and 0.01 % napthyl ethylene-dramine in distilled water, used after 12 hr of preparation) was added.
Each sample was treated with 50ul sulphanilamide
solution and incu-bated for 5 minutes at room temperature protecting from light.
at 30[degrees]C; 1mL of a 1%(w/v) solution of sulphanilamide
in 3 M HCl and 1 ml of a 0.02% (w/v) solution of Nepthyl Ethylene Diamine Dihydrochloride were added to the mixture; and the [A.sub.540] was measured.
To this, 60 [micro]d of Griess reagent A (1% sulphanilamide
in 1.2 M HCl) and then 60 [micro]l of Griess reagent B (0.3% N-[1-naphthyl]ethylenediamine) was added.
Fujie and Ogawa  determined sulphanilamide
by thermometric titrations.