supercoiling

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supercoiling

[′sü·pər‚kȯil·iŋ]
(cell and molecular biology)
Winding of the deoxyribonucleic acid duplex on itself so that it crosses its own axis; may be in the same (positive) direction as, or opposite (negative) direction to, the turns of the double helix.
References in periodicals archive ?
Since debye distance nearly equal to radius of DNA and supercoil assumed to be in ionic solution hence the effect of repulsion between the two strands at a distance s can be expressed in a dimensionless term as
Then they bend and twist again, folding into gnarled supercoils.
The size of the Supercoils makes them suitable for use in cooling metal-plating baths.
Furthermore, Top II-mediated DNA supercoil relaxation is stimulated by the Drosophila protein Barren [267], which is involved in chromosome condensation and disjunction [268].
Gel electrophoresis was used to study cleavage of supercoiled pUC18 DNA by the synthesized NPn1 and commercially available PLN.
The cleavage activity was demonstrated by gel-electrophoresis experiments using supercoiled (SC) plasmid pBR 322 DNA in a medium TAE buffer.
This results in accumulation of positively supercoiled DNA, which causes transcription and replication initiation failure with depletion of mitochondrial DNA copy number.
The CT-DNA binding and supercoiled pentry/d-topo plasmid DNA cleavage activities of copper complexes were also evaluated.
The experiment was accomplished with supercoiled pUC18 (200 ng, 1 [micro]L) which was treated with varying concentrations of compounds (10-50 [micro]M) in Trisboric acid-buffer with NaCl (50 mM, pH 7.2) by agarosegel electrophoresis method.
Top II plays a key role in relaxing supercoiled DNA for replication and transcription in the absence of inhibitors [38], while the presence of inhibitors forms a stable complex with the enzyme and keeps it from DNA cleavage [39].
Early publications related to observations of the DNA structure using AFM included imaging of single- and double-stranded DNA/RNA molecules [39-41], linear and circular DNA [42, 43], relaxed and supercoiled plasmids [44, 45], and the kDNA network of the trypanosomatid C.