Since debye distance nearly equal to radius of DNA and supercoil
assumed to be in ionic solution hence the effect of repulsion between the two strands at a distance s can be expressed in a dimensionless term as
Then they bend and twist again, folding into gnarled supercoils
The size of the Supercoils
makes them suitable for use in cooling metal-plating baths.
Furthermore, Top II-mediated DNA supercoil
relaxation is stimulated by the Drosophila protein Barren , which is involved in chromosome condensation and disjunction .
Gel electrophoresis was used to study cleavage of supercoiled
pUC18 DNA by the synthesized NPn1 and commercially available PLN.
The cleavage activity was demonstrated by gel-electrophoresis experiments using supercoiled
(SC) plasmid pBR 322 DNA in a medium TAE buffer.
This results in accumulation of positively supercoiled
DNA, which causes transcription and replication initiation failure with depletion of mitochondrial DNA copy number.
were monitored by conversion of supercoiled
plasmid pBR322 DNA to open circular form as described by Yeung et al.
The CT-DNA binding and supercoiled
pentry/d-topo plasmid DNA cleavage activities of copper complexes were also evaluated.
The experiment was accomplished with supercoiled
pUC18 (200 ng, 1 [micro]L) which was treated with varying concentrations of compounds (10-50 [micro]M) in Trisboric acid-buffer with NaCl (50 mM, pH 7.2) by agarosegel electrophoresis method.
Top II plays a key role in relaxing supercoiled
DNA for replication and transcription in the absence of inhibitors , while the presence of inhibitors forms a stable complex with the enzyme and keeps it from DNA cleavage .
Early publications related to observations of the DNA structure using AFM included imaging of single- and double-stranded DNA/RNA molecules [39-41], linear and circular DNA [42, 43], relaxed and supercoiled
plasmids [44, 45], and the kDNA network of the trypanosomatid C.