Among the Chinese population similar mutation was found to be significantly pathogenic causing familial hypercholesterolemia leading to premature coronary artery disease.22 One synonymous mutation
was detected at exon 2 in four patients.
Among the identified five SNPs, three SNPs were previously described, namely, synonymous mutation
c.183C>T and missense mutation c.748T>G by Fontanesi et al , and c.676A>G by Wu et al  and Javanmard et al .
To evaluate the implication of a novel synonymous mutation
, we used The Berkeley Drosophila Genome Project (http://www.fruitfly.org/) as a splice site prediction program.
And synonymous mutation
can be implicated in disease because of the effect on splicing and/or mRNA stability due to codon change as well as missense mutation.
[G.sup.12012] [right arrow] [A.sup.12012] change resulted in a synonymous mutation
at [L.sup.10] located within the predicted signal sequence of GP2, which was detected during sequential passages of DGQY1, and this nucleotide mutation also altered the amino acids mutation [D.sup.9] [right arrow] [N.sup.9] in E protein.
In one of them, no apparent mutation in katG, inhA were found (except in rpoB, where we found a synonymous mutation
), suggesting that there may be another DR mechanism.
Silent mutation at G779G found that though it was a synonymous mutation
the genotypic variations might influence the response to TKI as seen with Q787Q.
A sequence analysis of the gene encoding viral protein (VP)1 and of the combined region for VP2 and VP3 proteins revealed a previously undescribed synonymous mutation
in this isolate.
Coding position c.76 was found homozygous (G) in one CTVT sample (DP10) and heterozygous (C/G) in sample (DP3) and (C/T) heterozygous in another CTVT sample (DP6), which appeared as non synonymous mutation
and alter the leucine residue to valine in one case and phenylalanine in other case.
Two significant novel mutations that we identified in the duck DCT gene, a c.752A>G synonymous mutation
in exon 4 and the ce.938A>G SNP in exon 5, might have effects on plumage coloration as a consequence of the replacement of histidine with arginine, although further investigations using large populations are required to verify this.
Among the 22 unique mutations, there were 2 nonsynonymous mutations at position 2082 in the VP3 gene of both isolates and position 5037 in the 3A gene of SVA-OH1 and 1 unique synonymous mutation
only in SVA-OH2.
Although the G841A was a synonymous mutation
, not resulting in change of amino acid residue, it had remarkable effect on litter size in Jining Grey goat.