TRSV


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TRSV

McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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Los extractos de las muestras de las 20 plantas por parcela, se analizaron individualmente para la presencia de PVA, PVM, PVS, PLRV, PVX, PVY, APLV, PAMV, PVT, PVV y TRSV, utilizando el metodo DAS-ELISA ("double antibody sandwich enzyme-linked immunosorbent assay"), con anticuerpos de recubrimiento, anticuerpos conjugados con fosfatasa alcalina, controles positivos y negativos, y los protocolos de la casa comercial AGDIA (Elkhart, Indiana, USA).
El PVX se encontro en el 77 % de las muestras analizadas, seguido del PAMV con un 62 %, TRSV con un 42 %, PLRV 41 %, PVT 39 %, PVV 37 %, PMTV 31 %, PVY 30 %, PVS 19 %, PVM 13 %, APMoV 8 % y PVA en el 6 %.
El virus TRSV se detecto en el 70 % de las parcelas de las zonas media y alta, y en el 50 % en la zona baja.
Pedigree information was recorded for all cultivars cited as being BPMV, SMV, TRSV, or TSV-resistant.
Plants were inoculated with BPMV, SMV, TRSV, and TSV 7 to 10 d after planting at growth stage V1 (Fehr and Caviness, 1977).
Three weeks after inoculation, trifoliolate leaf samples were tested for the presence of BPMV, SMV, TRSV, and TSV by double antibody sandwich ELISA (Clark and Adams, 1977) using Agdia antibodies and protocol (Agdia, Inc., Elkhart, IN).
Evaluation of Ancestral Lines for Resistance to BPMV, SMV, TRSV, and TSV
All inoculated plants were ELISA-positive for TRSV. Symptoms caused by TRSV included stunted plants, distorted leaves, and bud blight, which consisted of a curving of the terminal bud that later became necrotic.
These results are in agreement with those of Arabidopsis thaliana (L.) Heynh., where genetic mapping studies revealed that TRSV resistance is controlled by a single major gene (Lee et al., 1996a).
Abbreviations: CIM, composite interval mapping; cM, centimorgan; LG, linkage group; MAS, market-assisted selection; QTL, quantitative trait locus; RFLP, restriction fragment length polymorphism; SMV, Soybean mosaic virus; SSR, simple sequence repeat; TRSV, Tobacco ringspot virus.