In the present study, the actin cortex layer around the dual contractile ring became thicker between anaphase to telophase
compared with that at metaphase, as described by Pielak et al.
However, the analyses of the Nesprin-2 depleted cells revealed the presence of chromatin bridges during ana- and telophase
. When we determined the chromatin bridges in cells transfected with SMC control and Ne-2 SMC shRNA at ana- and telophase
, we observed that 4.4% (mean value) of control cells harbored chromatin bridges.
Few aberrations were recorded in the interphase, prophase, and telophase
. Chromosomal aberrations such as bridges, fragments, binuclei, and micronucleus were observed and recorded (Figure 2).
With respect to the results listed in Table 2, the orange and grape juices of the five food companies, at both exposure times considered, induced significant formation of mitotic spindle changes, represented in this study by colchicine metaphase and anaphase and telophase
bridges, proving to be genotoxic, and chromosome breaks, characterized by the formation of micronuclei.
TABLE I NUCLEAR MATURATION OF BOVINE OOCYTES Total of Mature oocytes Immature oocytes evaluated oocytes MII+CP Telo I Total Ana I MI Total n n n n n n (%) (%) (%) (%) (%) (%) 172 95 26 121 2 46 48 (55.23) (15.11) (70.34) (116) (26.74) (27.9) Total of evaluated Degenerated oocytes oocytes n (%) 172 3 (174) Telol: Telophase
In metaphase, Aurora B is associated to the chromosomes, whereas in anaphase and telophase
it is localized to the midzone and midbody, respectively .
The late G2 phase, metaphase, and telophase
visualizations were obtained from treated zygotes at 21.0, 22.0, and 23.5 hpi, respectively.
The wrinkling revolution process is divided into five stages: (I) prophase stage, (II) prometaphase stage, (III) metaphase stage, (IV) anaphase stage, and (V) telophase
Microtubules formed arrays in the mitotic phase, including a preprophase band (PPB), the spindle apparatus at metaphase and anaphase, and the phragmoplast at telophase
, in the CK treatment (Fig.
2013) remains to be established, but the induction of p21 expression certainly contributed to the observed accumulation of cells in telophase
Oocytes that reached telophase
I or metaphase II stages were considered matured.
, the undamaged chromosomes, as well as the centric fragments, give rise to regular daughter nuclei.