To date, about 3000 mutations  have been detected in FBN1 and mainly classified into 3 types including missense mutations, in-frame deletions, and nonsense mutations due to frameshift leading to premature termination codons
(PTC) [4, 5], even though progresses had been made for the detection of causal mutations in MFS.
Among these mutations, seven can lead to premature termination codons
, two are point mutations, and two are small insertions.
Feature Mean value of Mean value of p value cassette exons constitutive exons Length 142.85 176.39 <2.2e -16 GC content 0.4975 0.5062 0.009886 Termination codon
0.0128 0.0117 0.0006005 5' splice strength -13.43874 -12.1145 0.0005285 3' splice strength -19.91228 -18.29848 0.0002665 Table 2: List of extracted features.
Introduction of a 30-nt deletion starting at the 212th position from the ORF termination codon
in the 3' NCR of DENV-4 reduced the ability of the virus to propagate in vivo and in vitro (16).
The NS3-Reverse primer (5'-AGAGAGGAATTCACTAGAATGGAAGCAGTAA-3') contains the 3' of the ORF (bases 717 to 734, in italics), the EcoR1 restriction site (bold) and the termination codon
(bold italics) (Fig.
Initiation and termination codons
were identified by comparison of the obtained genomes with fully sequenced O.
All amino acids specifying codons and the two termination codons
, TAA and TAG are used for O.
The [beta]-globin C[right arrow]G mutation at 6 by 3' to the termination codon
causes [beta]-thalassaemia by decreasing the mRNA level.
The size of introns in chicken [DELTA]-6 desaturase and the 5'splice donor and 3'splice acceptor (The exon includes initiation codon ATG and termination codon
TGA except 3'UTR and 5'UTR) Size 3' splice 5'splice Intron Exon (bp) acceptor donor size (bp) 1 207 CCACG/gtaag 3,374 2 111 tccag/GATGC AAAAT/gtgag 782 3 198 tgcag/TCCCA CCCAG/gtaag 644 4 102 ttcag/GCCCA TTAAG/gtaaa 229 5 126 cccag/GGTGC TTGAG/gtaag 2,003 6 61 cacag/TACGG CCTCA/gtgag 492 7 77 tgcag/TCTTC GGGCG/gtgag 212 8 98 cctag/GACCT GTCAG/gtagg 2,634 9 97 tgaag/GTTTC CTCAG/gtaat 1,137 10 80 tacag/CTGGC CACCA/gtgag 1,103 11 126 tcaag/CCTGT GTTGG/gtaag 526 12 52 cccag/GTCAC
These deletions overlap with the sequence palindrome (5'-TCCTCTTCTCCT-3'; c1925-1936), causing the same frame shift and a termination codon
after amino acid 643 in the mutant RET (Fig.
The Pakse (Ps) mutation involves an A [right arrow] T transversion in the termination codon
(Cd142) of the HBA2 gene, causing its conversion to tyrosine [TAA(ter) [right arrow] TAT(tyr)].
Although all possible changes except the conservative TGC to TGT and the TGC to the termination codon
TGA have been found in codon 634 in MEN 2A families, TGC (Cys) to TAC (Tyr) and TGC to CGC (Arg) are by far the most prevalent.