Methanol, n-hexane, dichloromethane, trimethylchlorosilane, hexamethyldisilazane, 1-decanol, sodium sulphate anhydrous, sodium carbonate, deuterated chloroform, and tetracosane
were from Sigma-Aldrich (Saint Louis, MO, USA), and hydrochloric acid and potassium hydroxide pellets were from Carlo Erba (Milan, Italy).
The derivatized samples were diluted with 600 [micro]L of a tetracosane
solution in hexane (0.01 mg/mL) just before GC-MS analysis.
Other authors also mention 1-octadecanol, 11-n-decyl tetracosane
(Romero-Lopez et al.
Due to the high boiling point of [C.sub.24][H.sub.50] (tetracosane
) at 391[degrees]C, there was no gaseous standard available for the program.
Aliphatic unbranched and branched long chain hydrocarbons from dodecane to tetracosane
form the last important compound group.
Among them the liquid alkanes (undecane dodecane tridecane tetradecane pentadecane hexadecane heptadecane etc.) are widely used in fuel oils and as anti-corrosive agents in lubricating oils and solid alkanes (octadecane nonadecane eicosane heneicosane tricosane tetracosane
heptacosane octacosane etc.) find their use in paraffin waxes of different kinds.
Sterol quantification was performed using tetracosane
(Sigma Aldrich) as the internal injection standard.
Patriscabratine and tetracosane
, isolated from the Bangladesh mangrove fern, Acrostichum aureum, reportedly showed moderately cytotoxic activity against AGS, MDA-MB-231 and MCF-7 cells, and AGS, MDA-MB-231, HT-29, and NIH 3T3 cells, respectively .
([C.sub.24][H.sub.50]) was used as an internal standard.
4) (Table 4): 1) Heneicosane (C21), 2) Linoleic acid (possible contaminat), 3) Docosane (C22), 4) Tricosane (C23), 5) Tetracosane
(C24), 6) Pentacosane (C25), C) phthalate 7) Hexacosane (C26), 8) Heptacosane (C27), 9) Octacosane (C28), 10) Nonacosane (C29), 11) Triacontane (C30) and 12) Hentriacontane (C31) and Dotriacontane (C32).
A mixture of saturated alkanes, such as tricosane, tetracosane
and pentacosane, among others, together with rotenone and naphthoquinones 2-acetyl-4H,9H -naphtho[2,3-b]furan-4,9-dione (10) and 2-(1-hydroxyethyl)-4H,9H-naphtho[2,3-b] furano-4,9-dione (11) (Figure 3), were isolated and identified from the hydro-alcoholic extract.
0.04 2301.6 diisooctyl ester Tetracosane
0.05 2359.7 Octacosane 0.34 2459.3 Heptacosane 0.11 nd Squalene 0.16 nd nd Squalene 0.16 nd nd, not determined; retention time of compounds superior to the retention time of the last FAME.