Thiochrome


Also found in: Medical.

Thiochrome

 

(C12H14ON4S), a product of the oxidation of thiamine in an alkaline medium. Thiochrome crystals are yellow. Aqueous solutions in ultraviolet light (λmax = 460–470 nm) are intensely fluorescent. A highly sensitive method of quantitative determination of thiamine and thiamine pyrophosphate (cocarboxylase) used in medical and biological research is based on the determination of the specific fluorescence of thiochrome.

References in periodicals archive ?
Jansen, "A chemical determination of aneurin (vitamin B1) by thiochrome reaction," Recueil des Travaux Chimiques des Pays-Bas, vol.
Brown, "Determination ofthiamine by the thiochrome method.
Leveille, "Modified thiochrome procedure for the determination of urinary thiamin," American Journal of Clinical Nutrition, vol.
Sauberlich, "Determination of urinary thiamin by the thiochrome method," Clinical Biochemistry, vol.
Stepuro, "Fluorescent properties of thiochrome in solvents of different polarity," Journal of Applied Spectroscopy, vol.
Haugen, "The determination of thiamine by the thiochrome method," Scandinavian Journal of Clinical and Laboratory Investigation, vol.
Caption: Figure 1: Photochemical dehydrogenation of thiamine (1) to thiochrome (3).
Caption: Figure 2: Absorption spectra of (a) thiamine HCl and (b) thiochrome, at pH 7.0.
Stability-Indicating Photochemical Method for the Assay of Thiamine by Spectrophotometry
Concentrations of thiochrome products stored in the dark at 10[degrees]C decreased by no more than5%in 48 h.
Determination of thiamine and thiamine phosphates in excitable tissues as thiochrome derivatives by reversed-phase high-performance liquid chromatography on octadecyl silica.
Rapid HPLC measurement of thiamine and its phosphate esters in whole blood
A reliable semi-automated method for the determination of total thiamin in whole blood by the thiochrome method with high performance liquid chromatography.
Vitamin [B.sub.1] status assessed by direct measurement of thiamin pyrophosphate in erythrocytes or whole blood by HPLC: comparison with erythrocyte transketolase activation assay
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