Samples were sent for cultivation on blood, chocolate, Mc-Conkey agar and thioglycolate
All blood specimens were put into thioglycolate
broth and subcultured after overnight incubation on 5% blood agar, Mac Conkey agar and Chocolate agar plates and incubated overnight at 37[degrees]C aerobically.
The medium used for isolation and cultivation of strains grown on Thioglycolate
Agar (TGA) medium and was prepared as follows; 15 g/L pancreatic digest of casein, 5.5 g/L dextrose, 5 g/L yeast extract, 2.5 g/L sodium chloride, 0.5 g/L sodium thioglycolate
, 0.5 g/L L-cystine, 1 mg/L resazurin, 75 g/L agar with final pH 7 (29).
The samples were cultured in thioglycolate
medium, Blood agar and MacConkey's agar to detect aerobic and other bacteria.
difficile culture, as follows (4): 2 g of each sample were incubated in thioglycolate
broth for seven days under anaerobic atmosphere.
It is safe, is free of thioglycolate
acid (the corrosive lash perm chemical that dehydrates lashes and makes them frizzy), and will make your beauty routine simple and easy.
perfringens grown in fluid thioglycolate
medium (FTH), which contains sodium thioglycolate
and L-cysteine .
broth tube was used for enrichment of anaerobes.
Secondly, 0.9 gram of dibutyltin bis-(isooctyl thioglycolate
) (1.5 wt% in comparison with PVC weight) was then added to the above mixture.
Fungal cultures grown in thioglycolate
broth were used as primary inoculums.
hydrochloric acid, waiting for thioglycolate
, precipitated at 4[degrees]C for 4 h and centrifuged at 10,000g for 10 min.