Immediately, after the solution was cooked, 6-10 drops of 1% thymolphthalein
indicator were added and the solution was immediately titrated with 0.1 N sodium hydroxide to the thymolphthalein
The alkaline phosphatase activity was measured based on the release of thymolphthalein
from the hydrolysis of thymolphthalein
The following Hammett indicators were utilized: phenolphthalein ([H.sub.-] = 8.2), thymolphthalein
([H.sub.-] = 10.0), 2,4-dinitroaniline ([H.sub.-] = 15.0), and 4-nitroaniline ([H.sub.-] = 18.4).
Rapid method for determination of alkaline phosphatase activity in human serum with thymolphthalein
The solution was then cooled to room temperature and titrated using thymolphthalein
as the indicator until a persistent color change was obtained.
Maternal blood placental alkaline phosphatase (PAP) activity was assayed by the Thymolphthalein
Monophosphate method (4) using the maternal sera samples heated at 65[degrees]C for 7 min.
This tendency was similar at the jejunum: 632; 1151; 749 and 405 ([micro]moles thymolphthalein
/min/mg protein) and ileum: 107; 225; 157 y 71 ([micro]moles thymolphthalein/min/mg protein) with the same concentrations of phytic phosphorus.
One strategy involves using an acid-base indicator such as thymolphthalein
, which has a deep blue color in an alkaline solution but is colorless in acidic conditions.
Analysis of breath tests: Expired [sup.14]C[O.sub.2] was collected through a calcium chloride column into a trapping agent consisting of 4 ml methylbenzethonium hydroxide and a pH colour indicator (thymolphthalein