test for sperm DNA integrity and elaboration of image cytometry algorithm.
(20) According to Messadi, "[The toluidine blue
staining] test appears to be highly sensitive (93.5 percent to 97.8 percent) but less specific (73.3 percent to 92.9 percent), mainly because of high false-positive results." (3,20,39) Toluidine blue
staining does have limitations, including selective staining which causes some premalignant and malignant lesions to go undetected.
Since fraction Cc-SP1 was weakly visualized on the gel by staining with toluidine blue
(Figure 2Aa), it was intended to more accurately analyze this SPs preparation using stains-all as another cationic dye for detection of glycans (Volpi & Maccari, 2002; Rodrigues et al., 2017b; Salles et al., 2017).
faecalis by using methylene blue and toluidine blue
as photosensitizers combined with NaOCl or sodium chloride and a 660 nm wavelength diode laser at 40 mW power as the light source.
Secondary lamellae from infected fish showed hyperplasia, bas, and tip thickening, and/or lamellar fusion (a-b: hematoxylin and eosin; c-d: Masson's trichrome); primary lamellae showed increased MCs/EGCs (arrows), monocytes (M), and lymphocytes (L) ssociated with connective tissue (e-f: Toluidine blue
The sensitivity and specificity of potassium permanganate and methylene blue was 100% as there were no false positive or false negative results whereas toluidine blue
, malachite green and blue ink showed few false negative results, malachite green with least sensitivity (88%) (Table 2).
According to our institute's protocol, staining the smears for 1 min with toluidine blue
followed by washing in tap water gave us satisfactory results for evaluating adequacy of the smears.
Positive MCs in the small intestine showed purple-red as they were modified with toluidine blue
staining; the light blue nucleus was located at the centre of cytoplasm and filled with dense purple particles.
Hand-sections were taken as close as possible to the area from which epidermal peels were taken, and then stained with Toluidine Blue
, a metachromatic stain.
Mast cell numbers were quantified using toluidine blue
staining and counting the number of toluidine blue
positive cells in 10 high-power field (h.p.f) under a 20x objective lens.
For academic interest, toluidine blue
staining was done, which showed typical metachromatism (Figure 7).
staining and safranin O staining were conducted for proteoglycan detection and extracellular matrix assessment following conventional methods.